Mechanism by which mechanical stimulation regulates chondrocyte apoptosis and matrix metabolism via primary cilia to delay osteoarthritis progression.
10.11817/j.issn.1672-7347.2025.250187
- Author:
Huixian LING
1
,
2
;
Sha WU
1
;
Ziyu LUO
1
;
Yuyan SUN
1
;
Hongwei SHEN
1
;
Haiqi ZHOU
1
;
Yuanyuan FU
1
;
Wen WANG
1
;
Thai Namanh NGO
1
;
Ying KONG
1
,
3
Author Information
1. Department of Rehabilitation Medicine, Second Xiangya Hospital, Central South University, Changsha
2. 218211119@csu.edu.cn.
3. kongying1502@csu.edu.cn.
- Publication Type:Journal Article
- Keywords:
cartilage;
intraflageller transport 88;
mechanical stimulation;
osteoarthritis;
primary cilia
- MeSH:
Animals;
Chondrocytes/cytology*;
Apoptosis/physiology*;
Mice;
Cilia/metabolism*;
Osteoarthritis/pathology*;
Extracellular Matrix/metabolism*;
Mice, Knockout;
Disease Progression;
Interleukin-1beta;
Male;
Cells, Cultured
- From:
Journal of Central South University(Medical Sciences)
2025;50(5):864-875
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:Osteoarthritis (OA) is one of the most common chronic degenerative diseases, with chondrocyte apoptosis and extracellular matrix (ECM) degradation as the major pathological changes. The mechanical stimulation can attenuate chondrocyte apoptosis and promote ECM synthesis, but the underlying molecular mechanisms remain unclear. This study aims to investigate the role of primary cilia (PC) in mediating the effects of mechanical stimulation on OA progression.
METHODS:In vivo, conditional knockout mice lacking intraflagellar transport 88 (IFT88flox/flox IFT88 knockout; i.e., primary cilia-deficient mice) were generated, with wild-type mice as controls. OA models were established via anterior cruciate ligament transection combined with destabilization of the medial meniscus, followed by treadmill exercise intervention. OA progression was evaluated by hematoxylin-eosin staining, safranin O-fast green staining, and immunohistochemistry; apoptosis was assessed by TUNEL staining; and limb function by rotarod testing. In vitro, primary articular chondrocytes were isolated from mice and transfected with lentiviral vectors to suppress IFT88 expression, thereby constructing a primary cilia-deficient cell model. Interleukin-1β (IL-1β) was used to induce an inflammatory environment, while cyclic tensile strain (CTS) was applied via a cell stretcher to mimic mechanical loading on chondrocytes. Immunofluorescence and Western blotting were used to detect the protein expression levels of type II collagen α1 chain (COL2A1), primary cilia, IFT88, and caspase-12; reverse transcription polymerase chain reaction was performed to assess COL2A1 mRNA levels; and flow cytometry was used to evaluate apoptosis.
RESULTS:In vivo, treadmill exercise significantly reduced Osteoarthritis Research Society International (OARSI) scores and apoptotic cell rates, and improved balance ability in wild-type OA mice, whereas IFT88-deficient OA mice showed no significant improvement. In vitro, CTS inhibited IL-1β-induced ECM degradation and apoptosis in primary chondrocytes; however, this protective effect was abolished in cells with suppressed primary cilia expression.
CONCLUSIONS:Mechanical stimulation delays OA progression by mediating signal transduction through primary cilia, thereby inhibiting cartilage degeneration and chondrocyte apoptosis.
