Salvianolic Acid B Exerts Antiphotoaging Effect on Ultraviolet B-Irradiated Human Keratinocytes by Alleviating Oxidative Stress via SIRT1 Protein.
10.1007/s11655-025-3931-9
- Author:
Qiao-Ju ZHANG
1
;
Xi LUO
2
;
Yu-Wen ZHENG
1
;
Jun-Qiao ZHENG
1
;
Xin-Ying WU
1
;
Shu-Mei WANG
1
;
Jun SHI
3
,
4
Author Information
1. School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, China.
2. College of Traditional Chinese Medicine, Huizhou Health Sciences Polytechnic, Huizhou, Guangdong Province, 516025, China.
3. School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, China. shijun8008@
4. com.
- Publication Type:Journal Article
- Keywords:
antiphotoaging;
oxidative stress;
salvianolic acid B;
silent information regulator 1
- MeSH:
Humans;
Sirtuin 1/metabolism*;
Ultraviolet Rays;
Oxidative Stress/radiation effects*;
Keratinocytes/metabolism*;
Molecular Docking Simulation;
Benzofurans/pharmacology*;
Skin Aging/radiation effects*;
Reactive Oxygen Species/metabolism*;
Cell Survival/radiation effects*;
HaCaT Cells;
Hydroxyproline/metabolism*;
Glutathione Peroxidase/metabolism*;
Catalase/metabolism*;
Depsides
- From:
Chinese journal of integrative medicine
2025;31(11):1021-1028
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore the anti-photoaging properties of salvianolic acid B (Sal B).
METHODS:The optimal photoaging model of human immortalized keratinocytes (HaCaT cells) were constructed by expose to ultraviolet B (UVB) radiation. The cells were divided into control, model and different concentrations of Sal B groups. Cell viability was measured via cell counting kit-8. Subsequently, the levels of oxidative stress, including reactive oxygen species (ROS), hydroxyproline (Hyp), catalase (CAT), and glutathione peroxidase (GSH-Px) were detected using the relevant kits. Silent information regulator 1 (SIRT1) protein level was detected using Western blot. The binding pattern of Sal B and SIRT1 was determined via molecular docking.
RESULTS:Sal B significantly increased the viability of UVB-irradiated HaCaT cells (P<0.05 or P<0.01). Sal B effectively scavenged the accumulation of ROS induced by UVB (P<0.05 or P<0.01). In addition, Sal B modulated oxidative stress by increasing the intracellular concentrations of Hyp and CAT and the activity of GSH-Px (P<0.05 or P<0.01). The Western blot results revealed a substantial increase in SIRT1 protein levels following Sal B administration (P<0.05). Moreover, Sal B exhibited good binding affinity toward SIRT1, with a docking energy of -7.5 kCal/mol.
CONCLUSION:Sal B could improve the repair of photodamaged cells by alleviating cellular oxidative stress and regulating the expression of SIRT1 protein.
