Buyang Huanwu Decoction Promotes Recovery after Spinal Cord Injury by Regulating cAMP/PKA/NF-κB p65 Pathway.
10.1007/s11655-025-4201-6
- Author:
Si-Yuan LI
1
;
Ting-Ting FAN
1
;
Jian YIN
2
;
Cai-Yun WAN
1
;
Mei-Li LI
2
;
Shuai-Shuai XIA
1
;
Qiang LI
3
;
Liang LI
4
,
5
Author Information
1. Provincial Key Laboratory of Traditional Chinese Medicine Diagnostics, Hunan University of Chinese Medicine, Changsha, 410208, China.
2. Department of Anatomy, Hunan University of Chinese Medicine, Changsha, 410208, China.
3. Department of Traditional Chinese Medicine, Hunan Brain Hospital, Changsha, 410007, China.
4. Provincial Key Laboratory of Traditional Chinese Medicine Diagnostics, Hunan University of Chinese Medicine, Changsha, 410208, China. superliliang@
5. com.
- Publication Type:Journal Article
- Keywords:
Buyang Huanwu Decoction;
Chinese medicine;
brain-derived neurotrophic factor;
cyclic adenosine monophosphate;
nuclear factor kappa-B;
protein kinase a;
spinal cord injury
- MeSH:
Animals;
Spinal Cord Injuries/pathology*;
Drugs, Chinese Herbal/therapeutic use*;
Rats, Sprague-Dawley;
Male;
Cyclic AMP/metabolism*;
Transcription Factor RelA/metabolism*;
Cyclic AMP-Dependent Protein Kinases/metabolism*;
Signal Transduction/drug effects*;
Brain-Derived Neurotrophic Factor/genetics*;
Red Nucleus/metabolism*;
Recovery of Function/drug effects*;
Neurons/metabolism*;
Rats
- From:
Chinese journal of integrative medicine
2025;31(7):635-643
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate whether Buyang Huanwu Decoction (BYHWD) had a good curative effect on the neuroprotection of red nucleus neurons after spinal cord injury (SCI) and the possible molecular mechanism.
METHODS:Ninety male Sprague-Dawley rats were divided into 5 groups (n=18 per group) according to a random number table, including the control, model, low- (12.78 g/kg, BL group), medium- (25.65 g/kg, BM group), and high-dose BYHWD groups (51.30 g/kg, BH group). A rubrospinal tract transection model in rats was established, and different doses of BYHWD were intragastrically administrated for 4 weeks. The forelimb locomotor function was recorded using the spontaneous vertical exploration test. Cyclic adenosine monophosphate (cAMP) level in red nucleus was detected through an enzyme-linked immunosorbent assay. The morphology and number of red nucleus neurons were observed using Nissl's staining and axonal retrograde tracing by Fluoro-Gold (FG). The expression of cAMP-dependent protein kinase A (PKA), nuclear factor kappa-B (NF-κB) p65, and brain-derived neurotrophic factor (BDNF) in red nucleus were detected using immunohistochemistry and quantitative real-time polymerase chain reaction.
RESULTS:Compared with the control group, the utilization rate of bilateral forelimbs, unilateral right forelimbs, proportion of FG-labeled positive neurons, cAMP level, protein expressions of PKA and BDNF, and BDNF mRNA expression were significantly decreased in the model group (P<0.01), while NF-κB p65 was increased in the model group (P<0.01). Compared with the model group, the utilization rate of bilateral forelimbs and unilateral right forelimbs were significantly higher in the BL, BM and BH groups (P<0.01), the proportion of FG-labeled positive neurons, cAMP level, protein expressions of PKA and BDNF and BDNF mRNA expression in all BYHWD groups were increased (P<0.05 or P<0.01), while NF-κB p65 were decreased in all BYHWD groups (P<0.05 or P<0.01).
CONCLUSIONS:BYHWD possesses a sound neuroprotective effect on red nucleus neurons after SCI, and the efficacy was dose-related. The mechanism may be related to regulating the cAMP/PKA/NF-κ B p65 signaling pathway, finally promoting expression of BDNF.
