Pien Tze Huang Attenuates Cell Proliferation and Stemness Promoted by miR-483-5p in Hepatocellular Carcinoma Cells.
10.1007/s11655-025-4126-0
- Author:
Li-Hui WEI
1
;
Xi CHEN
2
;
A-Ling SHEN
3
;
Yi FANG
1
;
Qiu-Rong XIE
1
;
Zhi GUO
3
;
Thomas J SFERRA
4
;
You-Qin CHEN
3
;
Jun PENG
5
Author Information
1. Innovation and Transformation Center, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
2. Department of Oncology, the 900th Hospital of the Chinese People's Liberation Army Joint Service Support Force, Fuzhou, 350003, China.
3. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
4. Department of Pediatrics, Rainbow Babies and Children's Hospital and Case Western Reserve University School of Medicine, Cleveland, OH, 44106, USA.
5. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. pjunlab@hotmail.com.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
Pien Tze Huang;
hepatocellular carcinoma;
miR-483-5p;
proliferation;
stemness
- MeSH:
Humans;
MicroRNAs/metabolism*;
Cell Proliferation/drug effects*;
Liver Neoplasms/drug therapy*;
Carcinoma, Hepatocellular/drug therapy*;
Hep G2 Cells;
Neoplastic Stem Cells/metabolism*;
Drugs, Chinese Herbal/therapeutic use*;
Gene Expression Regulation, Neoplastic/drug effects*
- From:
Chinese journal of integrative medicine
2025;31(9):782-791
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the effect of miR-483-5p on hepatocellular carcinoma (HCC) cells proliferation and stemness, as well as the attenuating effect of Pien Tze Huang (PZH).
METHODS:Differentially expressed miRNA between HepG2 cells and hepatic cancer stem-like cells (HCSCs) were identified by a miRNA microarray assay. miR-483-5p mimics were transfected into HepG2 cells to explore the effects of miR-483-5p on cell proliferation and stemness. HepG2 cells and HCSCs were treated with PZH (0, 0.25, 0.50 and 0.75 mg/mL) to explore the effects of PZH on the proliferation and stemness, both in non-induced state and the state induced by miR-483-5p mimics.
RESULTS:miR-483-5p was significantly up-regulated in HCSCs and its overexpression increased cell proliferation and stemness in HepG2 cells (P<0.05). PZH not only significantly inhibited proliferation in HepG2 cells, but also significantly suppressed the cell proliferation and self-renewal of HCSCs (P<0.05). The effects of miR-483-5p mimics on proliferation and stemness of HepG2 cells were partially abolished by PZH.
CONCLUSIONS:miR-483-5p promotes proliferation and enhances stemness of HepG2 cells, which were attenuated by PZH, demonstrating that miR-483-5p is a potential molecular target for the treatment of HCC and provide experimental evidence to support clinical use of PZH for patients with HCC.
