Qishen Granules Modulate Metabolism Flexibility Against Myocardial Infarction via HIF-1 α-Dependent Mechanisms in Rats.
10.1007/s11655-024-3667-y
- Author:
Xiao-Qian SUN
1
;
Xuan LI
1
;
Yan-Qin LI
1
;
Xiang-Yu LU
2
;
Xiang-Ning LIU
1
;
Ling-Wen CUI
3
;
Gang WANG
1
;
Man ZHANG
1
;
Chun LI
1
;
Wei WANG
4
,
5
Author Information
1. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China.
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100029, China.
3. Beijing Key Laboratory of Traditional Chinese Medicine Syndrome and Formula, Beijing University of Chinese Medicine, Beijing, 100029, China.
4. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China. wangwei26960@
5. com.
- Publication Type:Journal Article
- Keywords:
Qishen Granules;
energy metabolism;
heart failure;
hypoxia-inducible factor-1 α pathway;
substrate utilization
- MeSH:
Animals;
Myocardial Infarction/physiopathology*;
Male;
Hypoxia-Inducible Factor 1, alpha Subunit/metabolism*;
Rats, Sprague-Dawley;
Glucose/metabolism*;
Drugs, Chinese Herbal/therapeutic use*;
Energy Metabolism/drug effects*;
Rats;
Fatty Acids/metabolism*;
Myocardium/pathology*
- From:
Chinese journal of integrative medicine
2025;31(3):215-227
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To assess the cardioprotective effect and impact of Qishen Granules (QSG) on different ischemic areas of the myocardium in heart failure (HF) rats by evaluating its metabolic pattern, substrate utilization, and mechanistic modulation.
METHODS:In vivo, echocardiography and histology were used to assess rat cardiac function; positron emission tomography was performed to assess the abundance of glucose metabolism in the ischemic border and remote areas of the heart; fatty acid metabolism and ATP production levels were assessed by hematologic and biochemical analyses. The above experiments evaluated the cardioprotective effect of QSG on left anterior descending ligation-induced HF in rats and the mode of energy metabolism modulation. In vitro, a hypoxia-induced H9C2 model was established, mitochondrial damage was evaluated by flow cytometry, and nuclear translocation of hypoxia-inducible factor-1 α (HIF-1 α) was observed by immunofluorescence to assess the mechanism of energy metabolism regulation by QSG in hypoxic and normoxia conditions.
RESULTS:QSG regulated the pattern of glucose and fatty acid metabolism in the border and remote areas of the heart via the HIF-1 α pathway, and improved cardiac function in HF rats. Specifically, QSG promoted HIF-1 α expression and entry into the nucleus at high levels of hypoxia (P<0.05), thereby promoting increased compensatory glucose metabolism; while reducing nuclear accumulation of HIF-1 α at relatively low levels of hypoxia (P<0.05), promoting the increased lipid metabolism.
CONCLUSIONS:QSG regulates the protein stability of HIF-1 α, thereby coordinating energy supply balance between the ischemic border and remote areas of the myocardium. This alleviates the energy metabolism disorder caused by ischemic injury.
