Hepatoprotective Effect of Camel Thorn Polyphenols in Concanavalin A-Induced Hepatitis in Mice.
10.1007/s11655-024-3808-3
- Author:
Nageh Ahmed EL-MAHDY
1
;
Thanaa Ahmed EL-MASRY
1
;
Ahmed Mahmoud EL-TARAHONY
2
;
Fatemah A ALHERZ
3
;
Enass Youssef OSMAN
1
Author Information
1. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta, 31527, Egypt.
2. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta, 31527, Egypt. ahmedeltarahony2021@gmail.com.
3. Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia.
- Publication Type:Journal Article
- Keywords:
Alhagi maurorum ethanolic extract;
concanavalin A-induced hepatitis;
hepatoprotective effect
- MeSH:
Animals;
Concanavalin A;
Mice;
Polyphenols/pharmacology*;
Liver/drug effects*;
Plant Extracts/therapeutic use*;
Camelus;
Oxidative Stress/drug effects*;
Male;
Protective Agents/pharmacology*;
Chemical and Drug Induced Liver Injury/prevention & control*;
Apoptosis/drug effects*;
Hepatitis/pathology*;
Antioxidants/pharmacology*;
CD4-Positive T-Lymphocytes/drug effects*;
Inflammation Mediators/metabolism*
- From:
Chinese journal of integrative medicine
2024;30(12):1090-1100
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To explore the prophylactic and therapeutic effects of Alhagi maurorum ethanolic extract (AME) in concanavalin A (Con A)-induced hepatitis (CIH) as well as possible underlying mechanisms.
METHODS:Polyphenols in AME were characterized using high performance liquid chromatography (HPLC). Swiss albino mice were divided into 4 groups. Normal group received intravenous phosphate-buffered saline (PBS); Con A group received 40 mg/kg intravenous Con A. Prophylaxis group administered 300 mg/(kg·d) AME orally for 5 days before Con A intervention. Treatment group received intravenous Con A then administered 300 mg/kg AME at 30 min and 3 h after Con A intervention. After 24 h of Con A injection, hepatic injury, oxidative stress, and inflammatory mediators were assessed. Histopathological examination and markers of apoptosis, inflammation, and CD4+ cell infiltration were also investigated.
RESULTS:HPLC analysis revealed that AME contains abundant polyphenols with pharmacological constituents, such as ellagic acid, gallic acid, ferulic acid, methylgallate, and naringenin. AME alleviated Con A-induced hepatic injury, as manifested by a significant reduction in alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase (P<0.01). Additionally, the antioxidant effect of AME was revealed by a significant reduction in oxidative stress markers (nitric oxide and malondialdehyde) and restored glutathione (P<0.01). The levels of proinflammatory cytokines (tumor necrosis factor-α, interferon-γ, and interleukin-6) and c-Jun N-terminal kinase (JNK) activity were reduced (P<0.01). Histopathological examination of liver tissue showed that AME significantly ameliorated necrotic and inflammatory lesions induced by Con A (P<0.01). Moreover, AME reduced the expression of nuclear factor kappa B, pro-apoptotic protein (Bax), caspase-3, and CD4+ T cell hepatic infiltration (P<0.01). The expression of anti-apoptotic protein Bcl-2 was increased (P<0.01).
CONCLUSION:AME has hepatoprotective and ameliorative effects in CIH mice. These beneficial effects are likely due to the anti-inflammatory, antioxidant, and anti-apoptotic effects of the clinically important polyphenolic content. AME could be a novel and promising hepatoprotective agent for managing immune-mediated hepatitis.
