Brucea javanica Seed Oil Emulsion and Shengmai Injections Improve Peripheral Microcirculation in Treatment of Gastric Cancer.
10.1007/s11655-024-4103-z
- Author:
Li QUAN
1
;
Wen-Hao NIU
2
;
Fu-Peng YANG
2
;
Yan-da ZHANG
3
;
Ru DING
2
;
Zhi-Qing HE
2
;
Zhan-Hui WANG
2
;
Chang-Zhen REN
2
;
Chun LIANG
4
,
5
Author Information
1. Department of Cardiology, Shanghai Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, 200438, China.
2. Department of Cardiology, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 200003, China.
3. Department of Cardiology, Hospital of the 80th Group Army of PLA, Weifang, Shandong Province, 261021, China.
4. Department of Cardiology, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 200003, China. chunliangliang1985@
5. com.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
gene expression;
network pharmacology;
pathway analysis;
peripheral microvascular reactivity;
β-sitesterol
- MeSH:
Humans;
Microcirculation/drug effects*;
Drugs, Chinese Herbal/administration & dosage*;
Stomach Neoplasms/physiopathology*;
Emulsions;
Male;
Plant Oils/administration & dosage*;
Brucea/chemistry*;
Middle Aged;
Female;
Drug Combinations;
Human Umbilical Vein Endothelial Cells/metabolism*;
Seeds/chemistry*;
Injections;
Vascular Endothelial Growth Factor A/metabolism*;
Aged;
Network Pharmacology
- From:
Chinese journal of integrative medicine
2025;31(4):299-310
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore and verify the effect and potential mechanism of Brucea javanica Seed Oil Emulsion Injection (YDZI) and Shengmai Injection (SMI) on peripheral microcirculation dysfunction in treatment of gastric cancer (GC).
METHODS:The potential mechanisms of YDZI and SMI were explored through network pharmacology and verified by cellular and clinical experiments. Human microvascular endothelial cells (HMECs) were cultured for quantitative real-time polymerase chain reaction, Western blot analysis, and human umbilical vein endothelial cells (HUVECs) were cultured for tube formation assay. Twenty healthy volunteers and 97 patients with GC were enrolled. Patients were divided into surgical resection, surgical resection with chemotherapy, and surgical resection with chemotherapy combining YDZI and SMI groups. Forearm skin blood perfusion was measured and recorded by laser speckle contrast imaging coupled with post-occlusive reactive hyperemia. Cutaneous vascular conductance and microvascular reactivity parameters were calculated and compared across the groups.
RESULTS:After network pharmacology analysis, 4 ingredients, 82 active compounds, and 92 related genes in YDZI and SMI were screened out. β-Sitosterol, an active ingredient and intersection compound of YDZI and SMI, upregulated the expression of vascular endothelial growth factor A (VEGFA) and prostaglandin-endoperoxide synthase 2 (PTGS2, P<0.01), downregulated the expression of caspase 9 (CASP9) and estrogen receptor 1 (ESR1, P<0.01) in HMECs under oxaliplatin stimulation, and promoted tube formation through VEGFA. Chemotherapy significantly impaired the microvascular reactivity in GC patients, whereas YDZI and SMI ameliorated this injury (P<0.05 or P<0.01).
CONCLUSIONS:YDZI and SMI ameliorated peripheral microvascular reactivity in GC patients. β-Sitosterol may improve peripheral microcirculation by regulating VEGFA, PTGS2, ESR1, and CASP9.
