Morchella conica, Morchella esculenta and Morchella delicosa Induce Apoptosis in Breast and Colon Cancer Cell Lines via Pro-apoptotic and Anti-apoptotic Regulation.
10.1007/s11655-024-3819-0
- Author:
Faiz UL HAQ
1
;
Muhammad IMRAN
2
;
Sami ULLAH
3
;
Usman AFTAB
4
;
Tasleem AKHTAR
4
;
Asif Haleem KHAN
5
;
Roh ULLAH
5
;
Hasan EJAZ
6
;
Fatema GAFFAR
7
;
Imad KHAN
8
Author Information
1. Department of Microbiology, University of Health Sciences, Lahore, 54600, Pakistan.
2. Department of Microbiology, University of Health Sciences, Lahore, 54600, Pakistan. mi.bannu@yahoo.com.
3. Department of Forensic Sciences, University of Health Sciences, Lahore, 54600, Pakistan.
4. Department of Pharmacology, University of Health Sciences, Lahore, 54600, Pakistan.
5. Department of Hematology, University of Health Sciences, Lahore, 54600, Pakistan.
6. Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, 72388, Saudi Arabia.
7. Institute of Allied Health Sciences, De Montfort University, Leicester, LE1 9BH, UK.
8. State Key Laboratory of Food Science and Resources, National Engineering Research Center for Functional Food, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122, China.
- Publication Type:Journal Article
- Keywords:
Morchella species;
anticancer agent;
apoptosis;
breast cancer;
colon cancer;
fungal extracts;
mushrooms
- MeSH:
Humans;
Apoptosis/genetics*;
Colonic Neoplasms/drug therapy*;
Breast Neoplasms/drug therapy*;
Cell Line, Tumor;
Cell Proliferation/drug effects*;
Plant Extracts/pharmacology*;
Gene Expression Regulation, Neoplastic/drug effects*;
MCF-7 Cells;
Ascomycota/chemistry*
- From:
Chinese journal of integrative medicine
2025;31(10):918-927
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore the potential apoptotic mechanisms of 3 Morchella extracts (Morchella conica, Morchella esculenta and Morchella delicosa) on breast and colon cancer cell lines using apoptotic biomarkers.
METHODS:Human breast cell line (MCF-7) and colon cancer cell line (SW-480) were treated with methanol and ethanol extracts of 3 Morchella species with concentration ranging from 0.0625 to 2 mg/mL. After that their effects on gene expression of apoptosis related markers (pro-apoptotic markers including Bax, caspase-3, caspase-7, and caspase-9, and the antiapoptotic marker including Bcl-2) were determined using reverse transcription polymerase chain reaction.
RESULTS:All Morchella extracts reduced breast and colon cancer cells proliferation at half inhibitory concentration (IC50) of 0.02 ±0.01 to 0.68 ±0.30 mg/mL. As expected, all Morchella extracts significantly increased gene expressions of Bax, caspase-3, caspase-7, and caspase-9 and downregulated the gene expression of Bcl-2 in MCF-7 and SW-480 cell lines (P<0.05).
CONCLUSIONS:Morchella extracts demonstrated significant anti-proliferative activity against breast and colon cancer cell lines via an apoptosis induction mechanism. Anticancer activity of Morchella extracts and activation of apoptosis in breast and colon cancer cells suggest that it may be used to develop chemotherapeutic agents against cancer in future.
