Celastrus orbiculatus Extract Inhibits Immune Inflammatory Thrombotic State of B-Lymphoma.
10.1007/s11655-024-4102-0
- Author:
Miao ZHU
1
;
Qing-Qing SHI
2
;
Jun NI
2
;
Wei WU
2
;
Xing SUN
2
;
Mei SUN
1
;
Kai-Lin XU
3
;
Yan-Qing LIU
4
;
Jian GU
1
;
Hao GU
5
,
6
Author Information
1. Department of Hematology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu Province, 225001, China.
2. Yangzhou Institute of Hematology, Yangzhou, Jiangsu Province, 225001, China.
3. Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, 221000, China.
4. Department of Oncology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu Province, 225001, China.
5. Department of Neurology, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, 210000, China. guhao1980@
6. com.
- Publication Type:Journal Article
- Keywords:
Celastrus orbiculatus extract;
immune regulation;
lymphoma;
murine lymphoma cells;
proliferation inhibition;
thrombotic state;
tumor inflammation
- MeSH:
Animals;
Plant Extracts/pharmacology*;
Mice, Inbred BALB C;
Thrombosis/drug therapy*;
Celastrus/chemistry*;
Cell Line, Tumor;
Lymphoma, B-Cell/pathology*;
Apoptosis/drug effects*;
Inflammation/pathology*;
Cell Proliferation/drug effects*;
Mice;
Cell Cycle/drug effects*;
Male;
Cytokines/metabolism*;
Inflammation Mediators/metabolism*
- From:
Chinese journal of integrative medicine
2024;30(11):1018-1026
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the inhibitory effect of Celastrus orbiculatus extracts (COE) on the proliferation of lymphoma cells and the immune regulation ability on inflammation and thrombophilia in vivo.
METHODS:The 38B9 lymphoma cells were treated with COE (160 µ g/mL) and CTX (25 µ mol/L). The apoptosis rate and cell cycle of each group were detected by flow cytometry. The secretion of inflammatory factors, including interleukin (IL)-6, IL-10, and tumor necrosis factor α (TNF-α), in cell supernatant was detected by enzyme-linked immunosorbent assay (ELISA). In vivo, BALB/c mice were subcutaneously injected with 38B9 lymphoma cells to establish lymphoma model. COE (3 mg·kg-1·d-1) and CTX (40 mg·kg-1·d-1) were administered to the model mice, respectively. The expression of plasma inflammatory factors (IL-6, IL-10 and TNF-α) and thrombus indexes, including D-dimer (D-D), von Willebrand factor (vWF) and tissue factor (TF), were detected by ELISA before tumor bearing (1 d), after tumor formation (14 d) and after intervention (21 d). PicoGreen dsDNA was used to detect the level of serum neutrophil extracellular traps (NETs). Flow cytometry was used to detect the expression of platelet activation marker calcium-dependent lectin-like receptor 2 (CLEC-2). The tumor growth and survival of mice were recorded.
RESULTS:The 38B9 lymphoma cells were apoptotic after the intervention of COE and CTX. The ratio of G2-M phase cells decreased in COE intervented cells compared with the control cells (P<0.05), and S phase cells decreased in CTX intervented cells (P<0.05). Also, the secretion level of IL-6 was significantly reduced after COE or CTX intervention (P<0.05), and IL-10 was significantly increased (P<0.05). Furthermore, the tumor mass was reduced, and the median survival time was longer in COE and CTX intervented tumor-bearing mice than in non-intervented mice. The significantly lower levels of TNF-α, IL-6, NETs, TF, DD and CLEC-2, as well as higher IL-10 were observed in COE and CTX treatment mice in comparision with the control mice (P<0.05).
CONCLUSION:COE has a mild and stable anti-tumor effect, which can reduce the secretion of inflammatory factors by lymphoma cells and regulate thrombophilic state caused by tumor inflammatory microenvironment.
