Characteristics of N<sup>6</sup>-methyladenosine Modification During Sexual Reproduction of Chlamydomonas reinhardtii.

Ying LV; Fei HAN; Mengxia LIU; Ting ZHANG; Guanshen CUI; Jiaojiao WANG; Ying YANG; Yun-Gui YANG; Wenqiang YANG

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Characteristics of N⁶-methyladenosine Modification During Sexual Reproduction of Chlamydomonas reinhardtii.

Author: Ying LV ¹ ; Fei HAN ¹ ; Mengxia LIU ² ; Ting ZHANG ³ ; Guanshen CUI ³ ; Jiaojiao WANG ⁴ ; Ying YANG ⁵ ; Yun-Gui YANG ⁵ ; Wenqiang YANG ⁶
Author Information

1. Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; China National Botanical Garden, Beijing 100093, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
2. CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences and China National Center for Bioinformation, Beijing 100101, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China.
3. CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences and China National Center for Bioinformation, Beijing 100101, China.
4. Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; China National Botanical Garden, Beijing 100093, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China.
5. CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences and China National Center for Bioinformation, Beijing 100101, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China; Institute of Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.
6. Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; China National Botanical Garden, Beijing 100093, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China; Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100093, China. Electronic address: wqyang@ibcas.ac.cn.
Publication Type:Journal Article
Keywords: Chlamydomonas reinhardtii; Microtubule-associated pathway; N(6)-methyladenosine; Photosynthesis; Sexual reproduction; m(6)A sequencing
MeSH: Animals; Chlamydomonas reinhardtii/metabolism*; Reproduction/genetics*; Life Cycle Stages/genetics*; Transcriptome; Plants/genetics*
From: Genomics, Proteomics & Bioinformatics 2023;21(4):756-768
CountryChina
Language:English
Abstract: The unicellular green alga Chlamydomonas reinhardtii (hereafter Chlamydomonas) possesses both plant and animal attributes, and it is an ideal model organism for studying fundamental processes such as photosynthesis, sexual reproduction, and life cycle. N⁶-methyladenosine (m⁶A) is the most prevalent mRNA modification, and it plays important roles during sexual reproduction in animals and plants. However, the pattern and function of m⁶A modification during the sexual reproduction of Chlamydomonas remain unknown. Here, we performed transcriptome and methylated RNA immunoprecipitation sequencing (MeRIP-seq) analyses on six samples from different stages during sexual reproduction of the Chlamydomonas life cycle. The results show that m⁶A modification frequently occurs at the main motif of DRAC (D = G/A/U, R = A/G) in Chlamydomonas mRNAs. Moreover, m⁶A peaks in Chlamydomonas mRNAs are mainly enriched in the 3' untranslated regions (3'UTRs) and negatively correlated with the abundance of transcripts at each stage. In particular, there is a significant negative correlation between the expression levels and the m⁶A levels of genes involved in the microtubule-associated pathway, indicating that m⁶A modification influences the sexual reproduction and the life cycle of Chlamydomonas by regulating microtubule-based movement. In summary, our findings are the first to demonstrate the distribution and the functions of m⁶A modification in Chlamydomonas mRNAs and provide new evolutionary insights into m⁶A modification in the process of sexual reproduction in other plant organisms.

Characteristics of N6-methyladenosine Modification During Sexual Reproduction of Chlamydomonas reinhardtii.

Characteristics of N⁶-methyladenosine Modification During Sexual Reproduction of Chlamydomonas reinhardtii.