Screening Linear and Circular RNA Transcripts from Stress Granules.
10.1016/j.gpb.2022.01.003
- Author:
Shuai CHEN
1
;
Jinyang ZHANG
2
;
Fangqing ZHAO
3
Author Information
1. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
2. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China.
3. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, Kunming 650223, China; Key Laboratory of Systems Biology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310013, China. Electronic address: zhfq@biols.ac.cn.
- Publication Type:Letter
- Keywords:
Circular RNA;
Hepatocellular carcinoma;
Protein–RNA interaction;
RNA-seq;
Stress granule
- MeSH:
Animals;
Humans;
RNA, Circular/metabolism*;
Carcinoma, Hepatocellular/metabolism*;
Stress Granules;
Cytoplasmic Granules/metabolism*;
Liver Neoplasms/metabolism*;
RNA/metabolism*;
Stress, Physiological/genetics*;
Mammals/genetics*
- From:
Genomics, Proteomics & Bioinformatics
2023;21(4):886-893
- CountryChina
- Language:English
-
Abstract:
Stress granules (SGs) are cytoplasmic ribonucleoprotein assemblies formed under stress conditions and are related to various biological processes and human diseases. Previous studies have reported the regulatory role of some proteins and linear RNAs in SG assembly. However, the relationship between circular RNAs (circRNAs) and SGs has not been discovered. Here, we screened both linear RNAs and circRNAs in SGs using improved total RNA sequencing of purified SG cores in mammalian cells and identified circular transcripts specifically localized in SGs. circRNAs with higher SG-related RNA-binding protein (RBP) binding abilities are more likely to be enriched in SGs. Furthermore, some SG-enriched circRNAs are differentially expressed in hepatocellular carcinoma (HCC) and adjacent tissues. These results suggest the regulatory role of circRNAs in SG formation and provide insights into the biological function of circRNAs and SGs in HCC.
