Transcriptome-wide Dynamics of m6A mRNA Methylation During Porcine Spermatogenesis.
10.1016/j.gpb.2021.08.006
- Author:
Zidong LIU
1
;
Xiaoxu CHEN
1
;
Pengfei ZHANG
1
;
Fuyuan LI
1
;
Lingkai ZHANG
1
;
Xueliang LI
1
;
Tao HUANG
1
;
Yi ZHENG
1
;
Taiyong YU
1
;
Tao ZHANG
2
;
Wenxian ZENG
3
;
Hongzhao LU
4
;
Yinghua LV
5
Author Information
1. Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
2. School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China.
3. Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. Electronic address: zengwenxian2015@nwafu.edu.cn.
4. School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China. Electronic address: lhz780823@snut.edu.cn.
5. College of Chemistry and Pharmacy, Northwest A&F University, Yangling 712100, China. Electronic address: yinghua.lv@nwafu.edu.cn.
- Publication Type:Journal Article
- Keywords:
N(6)-methyladenosine;
Pig;
SETDB1;
Spermatogenesis;
Spermatogonial stem cell
- MeSH:
Animals;
Male;
Mice;
Cell Differentiation/genetics*;
Mammals/metabolism*;
Methylation;
RNA, Messenger/metabolism*;
Spermatogenesis/genetics*;
Spermatozoa/metabolism*;
Swine/genetics*;
Testis/metabolism*;
Transcriptome;
RNA Methylation/genetics*
- From:
Genomics, Proteomics & Bioinformatics
2023;21(4):729-741
- CountryChina
- Language:English
-
Abstract:
Spermatogenesis is a continual process that occurs in the testes, in which diploid spermatogonial stem cells (SSCs) differentiate and generate haploid spermatozoa. This highly efficient and intricate process is orchestrated at multiple levels. N6-methyladenosine (m6A), an epigenetic modification prevalent in mRNAs, is implicated in the transcriptional regulation during spermatogenesis. However, the dynamics of m6A modification in non-rodent mammalian species remains unclear. Here, we systematically investigated the profile and role of m6A during spermatogenesis in pigs. By analyzing the transcriptomic distribution of m6A in spermatogonia, spermatocytes, and round spermatids, we identified a globally conserved m6A pattern between porcine and murine genes with spermatogenic function. We found that m6A was enriched in a group of genes that specifically encode the metabolic enzymes and regulators. In addition, transcriptomes in porcine male germ cells could be subjected to the m6A modification. Our data show that m6A plays the regulatory roles during spermatogenesis in pigs, which is similar to that in mice. Illustrations of this point are three genes (SETDB1, FOXO1, and FOXO3) that are crucial to the determination of the fate of SSCs. To the best of our knowledge, this study for the first time uncovers the expression profile and role of m6A during spermatogenesis in large animals and provides insights into the intricate transcriptional regulation underlying the lifelong male fertility in non-rodent mammalian species.
