Investigation on the Role of Medical Recombinant Human-Derived Collagen Functional Dressings in Wound Healing.
10.12455/j.issn.1671-7104.250072
- Author:
Xiaoxiao GAI
1
;
Xiaoxia SUN
1
;
Wenqian MA
1
;
Zhenhua LIN
1
;
Xinyuan LI
1
;
Chenghu LIU
1
Author Information
1. Shandong Institute of Medical Device and Pharmaceutical Packing Inspection, Jinan,
- Publication Type:Journal Article
- Keywords:
cell migration assay;
medical recombinant human-derived collagen functional dressings;
neovascularization;
platelet-endothelial cell adhesion molecule;
recombinant type III humanized collagen;
wound healing
- MeSH:
Humans;
Wound Healing/drug effects*;
Recombinant Proteins/pharmacology*;
Animals;
Cell Proliferation;
Cell Movement;
Collagen/pharmacology*;
Vascular Endothelial Growth Factor A/metabolism*;
Bandages;
Platelet Endothelial Cell Adhesion Molecule-1/metabolism*;
Endothelial Cells;
Fibroblast Growth Factors/metabolism*
- From:
Chinese Journal of Medical Instrumentation
2025;49(4):415-422
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the biological effect of medical recombinant human-derived collagen functional dressings in wound healing.
METHODS:MTT assay and RTCA assay were used to detect cell toxicity and proliferation. Scratch assay and Transwell cell migration assay were used to detect cell motility and migration ability. Enzyme-linked immunosorbent assay was used to detect the contents of vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), and platelet-endothelial cell adhesion molecule (CD31) in the supernatant of four types of cells. After animal surgery, the surgical wound was taken at 1 week, 4 weeks and 13 weeks, respectively, for hematoxylin eosin (HE) staining and immunohistochemistry to observe the inflammatory response and CD31 expression of the wound.
RESULTS:Medical recombinant human-derived collagen functional dressing promotes cell proliferation and migration, enhances wound angiogenesis by upregulating the expression of VEGF, FGF, and CD31 in human dermal vascular endothelial cells (HDVEC) and human vascular endothelial cells (HVEC), thereby improving local blood supply to the wound, regulating the inflammatory response of the wound, and accelerating wound healing.
CONCLUSION:Recombinant type Ⅲ humanized collagen plays an important role in wound healing.
