Determination of Acetate Content in Hemodialysis Solutions and Dialysis Concentrates by HPLC.
10.12455/j.issn.1671-7104.240718
- Author:
Huadong WANG
1
;
Yue WANG
2
;
Dong LIU
3
;
Xianghui LIU
3
Author Information
1. Henan Center for Drug Evaluation and Inspection (Henan Center for Vaccine Inspection), Zhengzhou,
2. National Institutes for Food and Drug Control, Beijing,
3. Henan Institute for Drug and Medical Device Inspection, Zhengzhou,
- Publication Type:Journal Article
- Keywords:
acetate;
content;
dialysis concentrates;
high-performance liquid chromatography (HPLC)
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Acetates/analysis*;
Hemodialysis Solutions/analysis*;
Dialysis Solutions/analysis*;
Renal Dialysis
- From:
Chinese Journal of Medical Instrumentation
2025;49(3):350-354
- CountryChina
- Language:Chinese
-
Abstract:
This study establishes a high-performance liquid chromatography (HPLC) method for the determination of acetate content in hemodialysis solutions and dialysis concentrates. In this study, Synergi Polar-RP column is utilized. Phosphate buffered saline (50 mmol/L, pH=2.5) is used as a mobile phase. The flow rate is 1.0 mL/min. The wavelength of detection is 212 nm. Results show that the linear relationship of acetate is good in the range of 0.1~20 mmol/L, r =0.999 9 and the spike recoveries are from 98.9%~99.5%, RSD<0.5% ( n=3). This method can easily and accurately determine the acetate content in hemodialysis solutions and dialysis concentrates, and can be applied to quality control in the production and use of such products.
