Effects of LncRNA SNHG20 on epithelial mesenchymal transition and microtubule formation in human oral squamous cell carcinoma cells through targeted regulation of the miR-520c-3 p/RAB22A pathway
10.19723/j.issn.1671-167X.2025.01.005
- VernacularTitle:LncRNA SNHG20靶向调控miR-520c-3p/RAB22A通路对人口腔鳞状细胞癌细胞上皮间质转化及微管形成的影响
- Author:
Minying MA
1
;
Xiaoqin CHAO
1
;
Yang ZHAO
1
;
Guoting ZHAO
1
Author Information
1. 青海省第五人民医院,青海省肿瘤医院口腔科,西宁 810001
- Publication Type:Journal Article
- Keywords:
Oral squamous cell carcinoma;
Small nucleolar RNA host gene 20;
Micro RNA-520c-3p;
Rab protein 22a;
Epithelial mesenchymal transition;
Microtubule formation
- From:
Journal of Peking University(Health Sciences)
2025;57(1):26-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of LncRNA SNHG20 on epithelial mesenchymal transi-tion(EMT)and microtubule formation in human oral squamous cell carcinoma(OSCC)cells through targeted regulation of the miR-520c-3p/RAB22A pathway.Methods:After real-time fluorescence quanti-tative detection of LncRNA SNHG20,miR-520c-3p,RAB22A mRNA expression levels in OSCC tissues and cells,dual luciferase reporter assay was used to detect the relationship between the three.OSCC cells were randomly separated into control group,sh-NC group,sh-SNHG20 group,sh-SNHG20+anti NC group,and sh-SNHG20+anti miR-520c-3p group.Western blotting was used to detect the expres-sion of N-cadherin,vimentin,and E-cadherin proteins in the OSCC cells.The morphology of HSC-3 cells was observed under microscope.Changes in the number of microtubules formed were detected.The effect of LncRNA SNHG20 on the growth of OSCC tumors and the expression levels of LncRNA SNHG20,miR-520c-3p and RAB22 A in the transplanted tumors were detected by nude mice tumorigenesis experi-ment.Results:LncRNA SNHG20 and RAB22A mRNA were upregulated in the OSCC tissues and cells,while miR-520c-3p was downregulated(P＜0.05).There were binding sites between LncRNA SNHG20 and miR-520c-3p,RAB22A and miR-520c-3p,which had targeted regulation relationship.Compared with the sh-NC group,the sh-SNHG20 group had fewer stromal like cells,more epithelial like cells,incomplete microtubule structure,and fewer nodules.LncRNA SNHG20,RAB22A,N-Cadherin,and vimentin were downregulated,while miR-520c-3p and E-cadherin were upregulated(P＜0.05).Compared with the sh-SNHG20+anti-NC group,the sh-SNHG20+anti-miR-520c-3 p group had a higher number of stromal like cells,a lower number of epithelioid cells,tighter microtubule arrangement,and more micro-tubule nodules.miR-520c-3p and E-cadherin were downregulated,while RAB22A,N-cadherin,and vi-mentin were upregulated(P＜0.05).The transplanted tumor of OSCC in sh-SNHG20 group was smaller and lower than that in sh-NC group.The expression levels of LncRNA SNHG20 and RAB22A in the transplanted tumor tissues were lower than those in sh-NC group,and the expression level of miR-520c-3p was higher than that in sh-NC group(P＜0.05).Conclusion:LncRNA SNHG20 promotes epitheli-al-mesenchymal transition and microtubule formation in human oral squamous cell carcinoma cells by tar-geting the miR-520c-3p/RAB22A pathway.Inhibiting the expression of LncRNA SNHG20 can target and regulate the miR-520c-3p/RAB22A pathway to inhibit EMT and microtubule formation in OSCC cells.
