Cross-sectional biomonitoring of urinary deoxynivalenol, T-2 and HT-2 toxins, and zearalenone in Japanese adults.

Toshiki TAJIMA; Tomohiko ISOBE; Isao SAITO; Takaaki KONDO; Koji SUZUKI; Ryosuke FUJII; Yoshiki TSUBOI; Yoshiko SUGITA-KONISHI; Jun UEYAMA

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Cross-sectional biomonitoring of urinary deoxynivalenol, T-2 and HT-2 toxins, and zearalenone in Japanese adults.

Author: Toshiki TAJIMA ¹ ; Tomohiko ISOBE ² ; Isao SAITO ¹ ; Takaaki KONDO ¹ ; Koji SUZUKI ³ ; Ryosuke FUJII ³ ; Yoshiki TSUBOI ³ ; Yoshiko SUGITA-KONISHI ⁴ ; Jun UEYAMA ¹
Author Information

1. Department of Biomolecular Sciences, Field of Omics Health Sciences, Nagoya University Graduate School of Medicine.
2. Health and Environmental Risk Division, National Institute for Environmental Studies.
3. Department of Preventive Medical Sciences, Fujita Health University School of Medical Sciences.
4. Faculty of Applied Biosciences, Tokyo University of Agriculture.
Publication Type:Journal Article
Keywords: Human biomonitoring; Japanese adults; Liquid chromatography–tandem mass spectrometry; Mycotoxin; Urine
MeSH: Humans; Zearalenone/urine*; Japan; Male; Female; Trichothecenes/urine*; T-2 Toxin/urine*; Biological Monitoring; Adult; Middle Aged; Aged; Cross-Sectional Studies; Young Adult; Mycotoxins/urine*; Environmental Exposure/analysis*; East Asian People
From:Environmental Health and Preventive Medicine 2025;30():19-19
CountryJapan
Language:English
Abstract: BACKGROUND:Among the more than 300 mycotoxins that are known to have toxic effects on animals and humans, Fusarium toxins deoxynivalenol (DON), T-2 and HT-2 toxins (T2/HT2), and zearalenone (ZEN) are frequently detected in domestic agricultural products. This study aimed to assess DON, T2/HT2, and ZEN exposure in Japanese adults by measuring urinary mycotoxins, observing their distributions, and making comparisons with data from other countries.
METHODS:A total of 201 individuals participated in the study. Twenty-four-hour urine samples were collected from young adults (34 men and 35 women) in the Tokai region (urban area) and spot urine samples were collected from middle-aged and elderly adults (64 men and 68 women) in the Donan area of Hokkaido Prefecture (rural area). Urinary DON, T2/HT2, and ZEN levels were measured using a validated liquid chromatography-tandem mass spectrometry method.
RESULTS:For DON, T2/HT2, and ZEN, the detection frequencies above the limit of detection (LOD) level (0.15, 0.13, and 0.01 µg/L, respectively) in all the samples were 53%, 26%, and 71%, respectively. The median concentrations (95^th percentile) of urinary DON, HT2, and ZEN were 0.19 (3.93),
CONCLUSIONS:This study represents the first comprehensive exposure assessment for DON, T2/HT2, and ZEN in Japanese adults using human biomonitoring methods. These data provide valuable information for a better understanding of mycotoxin exposure in Japan.