Expression and function of CDYL-interacting protein MYH9 in mouse testis
10.13263/j.cnki.nja.2025.09.001
- VernacularTitle:小鼠睾丸中CDYL相互作用蛋白MYH9的表达及功能初探
- Author:
Huan-tong GONG
1
;
Yan-mei QUAN
;
Yun-xia ZHANG
;
Han-fei ZHU
;
Xiao-yu XIA
Author Information
1. 上海交通大学医学院组织胚胎学与遗传发育学系/上海市生殖医学重点实验室,上海 200025
- Publication Type:Journal Article
- Keywords:
CDYL;
MYH9;
spermiogenesis;
manchette;
oligoasthenoteratozoospermia
- From:
National Journal of Andrology
2025;31(9):771-779
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the CDYL-interacting proteins in murine testis and investigate the mechanism of CDYL in-volved in spermatogenesis.Methods:CDYL-interacting partners in testis were identified using co-immunoprecipitation coupled with liquid chromatography-tandem mass spectrometry(LC-MS/MS).Expression pattern of CDYL-interacting protein MYH9 was analyzed through immunohistochemistry(IHC),confocal immunofluorescence(IF)and Western blot(WB)in mouse testicular cells.The effect of the Cdyl conditional knockout(CdylcKO)in spermatogenic cell on Myh9 expression was quantified via RT-qPCR,WB and IF imaging in both spermatids and spermatozoa from cauda epididymides.Results:Direct interaction between MYH9 and CDYL was confirmed in murine testis.During spermiogenesis,MYH9 exhibited co-localization with CDYL at the manchette structure,and binding to F-ACTIN,the component of manchette.In cauda epididymal spermatozoa,MYH9 signal concentrated on acrosomal region and con-tinuously distributed along the tail length.Conditional deletion of Cdyl in spermatogenic cell resulted in the transcriptional downregula-tion of Myh9.In spermatids,CdylcKO led to reduced but retained MYH9 localization to the disorganized manchette structure.In sperma-tozoa from CdylcKO mice,abnormalities of MYH9 localization were observed,including attenuation of acrosomal signal and/or partial vanishment/enhancement of tail signal.Conclusion:In murine spermatids,MYH9 protein is localized to the manchette structure,with its expression and subcellular distribution is affected by CDYL protein.CDYL-MYH9 interaction is essential for the spermiogenesis.
