Qiwei No.3 combined with sildenafil inhibits Rho kinase activity and increases AKT/eNOS expressions in the penile cavernosum of rats with diabetic erectile dysfunction.
- Author:
Wei ZHAO
1
;
Lin LI
1
;
Li ZHANG
1
;
Xiao-Qing ZHAO
1
;
Dong-Xu LI
1
;
Jing XIA
1
Author Information
1. Department of Andrology, Yangzhou Traditional Chinese Medicine Hospital, Yangzhou, Jiangsu 225000, China.
- Publication Type:Journal Article
- Keywords:
diabetic erectile dysfunction;
Qiwei No.3;
Rho kinase;
p-AKT protein;
eNOS protein
- MeSH:
Animals;
Male;
Sildenafil Citrate;
Rats;
rho-Associated Kinases/antagonists & inhibitors*;
Rats, Sprague-Dawley;
Penis/drug effects*;
Erectile Dysfunction/etiology*;
Proto-Oncogene Proteins c-akt/metabolism*;
Nitric Oxide Synthase Type III/metabolism*;
Diabetes Mellitus, Experimental/complications*;
Drugs, Chinese Herbal/therapeutic use*
- From:
National Journal of Andrology
2024;30(12):1128-1134
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of Qiwei No.3 combined with sildenafil on Rho kinase activity and AKT/eNOS pathways in the penile cavernous tissue of male rats with diabetic erectile dysfunction (DED).
METHODS:We constructed a model of DED in 24 SD male rats by intraperitoneal injection of streptozotocin solution and injecting apomorphine into the neck after 8 weeks of feeding, equally randomized the model rats into a model control (MC), a sildenafil (S), a low-dose Qiwei No.3 combined with sildenafil (LQ+S) and a high-dose Qiwei No.3 combined with sildenafil (HQ+S) group, and took another 6 normal male rats as blank controls (BC). We treated intragastrically the animals in the BC and MC groups with normal saline, and those in the S, LQ+S and HQ+S groups with sildenafil (5 mg/kg/d), Qiwei No.3 (10 g/kg/d) + sildenafil (5mg/kg/d), and Qiwei No.3 (20g/kg/d) + sildenafil (5mg/kg/d), respectively. After 6 weeks of treatment, we recorded the number of penile erections of all the rats by injecting apomorphine into the neck, and measured the activity of Rho kinase and expressions of p-AKT and eNOS proteins in the corpus cavernosum by Western blot.
RESULTS:Compared with the blank controls, all the DED model rats showed evidently elevated blood glucose and reduced body weight. The number of penile erections was significantly increased in the S, LQ+S and HQ+S groups in comparison with that in the model controls (P< 0.05), even higher in the HQ+S than in the S group (P< 0.05). The activity of Rho kinase in the penile cavernosum was significantly higher in the MC than in the BC group (P<0.05), but lower in the HQ+S than in the S group (P< 0.05). No statistically significant difference was observed in the expression level of the p-AKT protein in the penile cavernosum among the five groups of rats (P > 0.05). The expression of eNOS was remarkably up-regulated in the BC and HQ+S groups (P< 0.05) compared with that in the MC group, even more significantly in the HQ+S than in the LQ+S and S groups (P< 0.05).
CONCLUSION:The combination of high-dose "Qiwei No. 3" and sildenafil can improve erectile function in DED rats, which may be attributed to its effect of releasing more nitric oxide (NO) by inhibiting the activity of Rho kinase and up-regulating the expression of the e-NOS protein.
