The Role of Sema4D in Immune Abnormalities Mediated by IgA Secreted by B Lymphocytes in Children with Henoch-Schonlein Purpura.
10.19746/j.cnki.issn.1009-2137.2025.05.036
- Author:
Dan SU
1
;
Liu-Ming SUN
1
;
Wan-Hui LI
1
;
Xiao-Qian LYU
1
Author Information
1. Pediatric Department of Hengshui People's Hospital, Hengshui 053000, Hebei Province, China.
- Publication Type:Journal Article
- Keywords:
semaphorin 4D;
children;
Henoch-Schonlein purpura;
B lymphocyte;
immunoglobulin A
- MeSH:
Humans;
IgA Vasculitis/immunology*;
Semaphorins/metabolism*;
B-Lymphocytes/metabolism*;
Immunoglobulin A/immunology*;
Child;
Antigens, CD/metabolism*;
Male;
Female;
Child, Preschool
- From:
Journal of Experimental Hematology
2025;33(5):1486-1490
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the role of semaphorin 4D (Sema4D) in immunoglobulin A (IgA) -mediated immune abnormalities in B lymphocytes of pediatric Henoch-Schonlein purpura (HSP).
METHODS:One hundred HSP children admitted to Hengshui People's Hospital from January 2022 to January 2023 were selected as HSP group, and one hundred healthy children as control group. Sema4D expression was detected, and the relationship between Sema4D expression in children's serum and skin lesions and clinical characteristics of children was analyzed. Sema4D expression on the surface of lymphocytes of HSP children was detected. Different concentrations of human recombinant Sema4D protein was used to stimulate peripheral blood mononuclear cells in HSP children in vitro. The expression level of IgA in the supernatant was detected to verify whether Sema4D mediates immune abnormalities through IgA secreted by B lymphocytes.
RESULTS:The Sema4D level in the HSP group was significantly higher than that in the control group (P <0.001). Sema4D level in HSP children with severe, renal involvement, and joint involvement was higher than those with mild to moderate disease, and no renal or joint involvement (all P <0.001). Compared with control group, IgA level, CD8 + T lymphocyte proportion, and CD19 + B lymphocyte proportion in the HSP group were significantly higher but CD4 + T lymphocyte proportion was lower (all P <0.001). The expression levels of Sema4D on the surface of CD4 + T lymphocytes, CD8 + T lymphocytes, and CD19 + B lymphocytes in the HSP group were significantly higher than those in the control group (all P <0.001). With the increase of human recombinant Sema4D protein concentration, the level of IgA expression in HSP children gradually increased (P <0.05). Correlation analysis showed that Sema4D was significantly positively correlated with IgA (r =0.667).
CONCLUSION:HSP children show high expression of Sema4D, especially on the surface of T and B lymphocytes. The shedding of Sema4D from membrane surface may stimulate B lymphocytes to secrete IgA by binding to CD72, leading to immune abnormalities.
