Effects of Total Body Irradiation with 60 Co Gamma Ray at Different Dose Rates on Hematopoietic and Immune Cells in Mice.
10.19746/j.cnki.issn.1009-2137.2025.04.038
- Author:
Hui SHU
1
;
Ya DONG
2
;
Xue-Wen ZHANG
3
;
Xing SHEN
3
;
Shuang XING
3
;
Zu-Yin YU
1
Author Information
1. School of Life Sciences, Anhui Medical University, Hefei 230032, Anhui Province, China.
2. Graduate School of Jinzhou Medical University, Jinzhou 121001, Liaoning Province, China.
3. Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- Keywords:
irradiation dose rate;
gamma radiation;
hematopoietic cell;
immune cell
- MeSH:
Animals;
Mice;
Whole-Body Irradiation;
Gamma Rays;
Mice, Inbred C57BL;
Hematopoietic Stem Cells/radiation effects*;
Cobalt Radioisotopes;
Dose-Response Relationship, Radiation;
Male
- From:
Journal of Experimental Hematology
2025;33(4):1181-1189
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of irradiation dose rate of 60Co γ-ray on hematopoietic and immune cells in total body irradiation (TBI) mice.
METHODS:After TBI with 8 Gy 60Co γ-ray at three irradiation dose rates of 0.027, 0.256 and 0.597 Gy/min, the survival and change of body weight of C57BL/6J mice were observed within 30 days. The peripheral blood parameters were examined at each time point within 30 days post-irradiation. The hematopoietic stem/progenitor cell counts of mice were examined on the 10th and 30th day post-irradiation by flow cytometry, as well as the proportions of immune cells in peripheral blood, bone marrow and spleen of mice on the 30th day post-irradiation.
RESULTS:After TBI with 8 Gy 60Co γ-ray, the 30-day survival rate of high dose-rate group was 0, which was significantly lower than 90% of medium dose-rate group and 100% of low dose-rate group (both P < 0.001). The peripheral blood parameters of all three groups showed a sharp decline → low value → gradually recovering trend. The count of white blood cell, neutrophil, lymphocyte, red blood cell, platelet and hemoglobin level in the high dose-rate and medium dose-rate groups were significantly lower than those in the low dose-rate group on day 7-18 post-irradiation (all P < 0.05), but there were no significant differences between the high dose-rate and medium dose-rate groups (P >0.05). On the 10th day after irradiation, the proportion and number of bone marrow hematopoietic stem/progenitor cells (including LK, LSK, LT-HSC, ST-HSC, and MPP cells) in the low dose-rate and medium dose-rate groups were significantly decreased compared to those in the normal group (all P < 0.05), but there were no significant differences between the two groups (P >0.05). On the 30th day after irradiation, LSK, LT-HSC, ST-HSC and MPP cells in the low dose-rate group recovered to normal levels, while those in the medium dose-rate group were still significantly lower than those in the low dose-rate group (all P < 0.001). The results of bone marrow and peripheral immune cell tests on the 30th day after irradiation showed that the ratios of T and B lymphocytes in the low dose-rate and medium dose-rate groups were reduced compared to that in the normal group (both P < 0.05), while the ratio of neutrophils was increased (P < 0.01). The trend of changes in the spleen and peripheral blood was consistent.
CONCLUSION:The degree of hematopoietic and immune cell damage in mice after TBI with 8 Gy 60Co γ-ray is related to the dose rate, and low dose-rate irradiation can reduce the damage in the animal model. Therefore, choosing the appropriate dose rate of irradiation is a key factor in establishing an objective and reliable experimental animal model of irradiation.
