Correlation Analysis between Immune Cells in Graft and Early Immune Reconstitution after Allogeneic Hematopoietic Stem Cell Transplantation.
10.19746/j.cnki.issn.1009-2137.2025.04.037
- Author:
Shan WANG
1
;
Fan LIU
1
;
Qiu-Juan ZHU
1
;
Tao WANG
1
;
Rong GONG
1
;
Wei-Wei TIAN
1
;
Zhi-Lin GAO
1
Author Information
1. Department of Hematopathology, The Third Hospital of Shanxi Medical University (Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital), Taiyuan 030032, Shanxi Province, China.
- Publication Type:Journal Article
- Keywords:
allogeneic hematopoietic stem cell transplantation;
graft;
immune cells;
lymphocyte;
immune reconstitution
- MeSH:
Humans;
Hematopoietic Stem Cell Transplantation;
Immune Reconstitution;
Transplantation, Homologous;
Retrospective Studies;
Graft vs Host Disease/immunology*;
Male;
Female;
Killer Cells, Natural/immunology*;
Adult;
Middle Aged;
B-Lymphocytes/immunology*;
Prognosis;
Lymphocyte Subsets/immunology*;
Adolescent
- From:
Journal of Experimental Hematology
2025;33(4):1173-1180
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the correlation between the types and quantities of immune cells in the graft and early immune reconstitution after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and their influence on clinical prognosis.
METHODS:The clinical data of 83 patients with hematological diseases who received allo-HSCT in Shanxi Bethune Hospital from September 2020 to June 2023 were retrospectively analyzed. The number of mononuclear cells (MNC), CD34+ cells and lymphocyte subsets (including CD3+T, CD3+CD4+T(Th), CD3+CD8+T(Ts), NK cells and B cells) infused into the recipients was counted, and the peripheral blood lymphocytes were detected before conditioning and on days 14, 30, 60 and 100 post-HSCT.
RESULTS:Multivariate analysis showed that the number of MNC in the graft affected the recovery of CD4+T lymphocytes after HSCT, and the number of CD4+T lymphocytes in the graft affected the recovery of NK cells and B cells after HSCT. The patient age, donor sex, stem cell source, degree of HLA matching, use of ATG before HSCT, the occurrence of acute graft-versus-host disease (aGVHD) after HSCT, and viral infection all affect the early cellular immune reconstitution post-HSCT. The number of infused cells had no significant impact on the median engraftment time for neutrophils and platelets after HSCT. Patients with lower numbers of CD3+T, CD4+T and B cells in the graft were more prone to viral infection after HSCT. However, the cells in the graft had no significant effect on disease recurrence or mortality.
CONCLUSION:The recovery rate of lymphocyte count after allo-HSCT varies. The numbers of MNC and CD4+T cells in the graft may be related to the cellular immune reconstitution after HSCT, while the numbers of CD34+,CD3+T,CD8+T,NK and B cells have no significant effect on the cellular immune reconstruction. The numbers of CD3+T,CD4+T and B cells in the graft were negatively correlated with viral infection after HSCT, but the cellular components of the graft have no obvious influence on hematopoietic reconstitution, disease recurrence, death, recurrence-free survival(RFS) and overall survival(OS) after HSCT.
