A Screening Study of GP.Mur Antigen in Blood Donors in Jiangsu Region.
10.19746/j.cnki.issn.1009-2137.2025.04.033
- Author:
Lei SHAO
1
;
Tai-Xiang LIU
1
;
Ling MA
1
;
Fang ZHAO
1
;
Ruo-Yang ZHANG
1
;
Hong LIN
1
Author Information
1. Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China.
- Publication Type:Journal Article
- Keywords:
blood donor;
GP.Mur antigen;
PCR-SSP;
blood transfusion safety
- MeSH:
Humans;
Blood Donors;
China;
Genotype;
Blood Group Antigens/genetics*;
Polymerase Chain Reaction;
Glycophorins/genetics*;
Gene Frequency
- From:
Journal of Experimental Hematology
2025;33(4):1150-1154
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the distribution of GP.Mur antigen in blood donors in Jiangsu Province.
METHODS:Genomic DNA was extracted from 1 114 blood donors in Jiangsu region. PCR-SSP was performed to amplify GP.Mur, and gene analysis was conducted by direct sequencing of the PCR products. The frequency of GP.Mur in the blood donor population of Jiangsu region was calculated.
RESULTS:Out of 1 114 randomly selected blood samples, 11 positive bands were detected during amplification. Direct sequencing analysis revealed that among the 11 positive samples, 4 were homozygous for GYP .Mur genotype, 3 were heterozygous for GYP .Mur genotype, and the remaining 4 samples were identified as GYP .HF genotype.
CONCLUSION:This study analyzed the distribution of the GP.Mur antigen and preliminary obtained the frequency data in the blood donor population in Jiangsu region. Further in-depth research on this blood group is of great importance in guiding clinical blood transfusion practices and ensuring transfusion safety.
