Analysis of Correlation between Platelet Desialylation,Apoptosis and Platelet Alloantibody and CD8+T Cells in Platelet Transfusion Refractoriness
10.19746/j.cnki.issn1009-2137.2025.04.031
- VernacularTitle:血小板输注无效患者中的去唾液酸化、凋亡现象与抗体特异性及CD8+T细胞的相关性分析
- Author:
Yan ZHOU
1
;
Li-Yang LIANG
1
;
Chang-Shan SU
1
;
Hui-Hui MO
1
;
Ying CHEN
1
;
Fang LU
1
;
Yu-Chen HUANG
1
;
Zhou-Lin ZHONG
1
Author Information
1. 南宁中心血站(南宁输血医学研究所),广西南宁 530007
- Publication Type:Journal Article
- Keywords:
PTR;
desialylation;
apoptosis;
alloantibody;
CD8+T cell
- From:
Journal of Experimental Hematology
2025;33(4):1138-1144
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the correlation between platelet alloantibodies and CD8+T cell with platelet desialylation and apoptosis in platelet transfusion refractoriness(PTR).Methods:The expression of RCA-1,CD62P and Neu1 on platelets were detected in 135 PTR patients and 260 healthy controls.The ability of PTR patients'sera with anti-HLA antibody,anti-CD36 antibody and antibody-negative groups to induce platelet desialylation and apoptosis,and the potential effect of FcγR inhibitors on desialylation and apoptosis were evaluated.Additionally,the association between CD8+T cells and platelet desialylation in patients was analyzed.Results:The expression of RCA-1 and Neu1 on platelets in PTR patients were significantly higher than those in healthy donors(P＜0.05),but were not related to platelet alloantibody(P＞0.05).The sera of PTR patients generally induced platelet desialylation in vitro(P＜0.05),with no significant differences among the groups(P＞0.05).However,the sera with anti-CD36 antibodies could induce platelet apoptosis significantly higher than that in the anti-HLA antibody group and antibody-negative group in vitro(P＜0.05).In PTR patients with anti-CD36 antibodies,platelet apoptosis was dependent on FcγR signaling,while desialylation is not.Moreover,CD8+T cells in PTR patients were significantly associated with platelet desialylation(P＜0.05).Conclusion:Platelet desialylation is a common pathological phenomenon in PTR patients,which involves the participation of CD8+T cell,but isn't associated with platelet alloantibody;while anti-CD36 antibodies have potential clinical significance in predicting platelet apoptosis in PTR patients.
