Expression of CSF-1/CSF-1R in the Peripheral Blood of Children with Immune Thrombocytopenia and Its Clinical Significance.
10.19746/j.cnki.issn.1009-2137.2025.04.030
- Author:
Dan-Lu LI
1
;
Hai-Chen SONG
1
;
Yong-Feng CHENG
1
;
Mei YAN
1
Author Information
1. First Department of Internal Pediatrics, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China.
- Publication Type:Journal Article
- Keywords:
CSF-1/CSF-1R;
immune thrombocytopenia;
macrophage polarization;
M1/M2 imbalance
- MeSH:
Humans;
Purpura, Thrombocytopenic, Idiopathic/blood*;
Macrophage Colony-Stimulating Factor/metabolism*;
Leukocytes, Mononuclear/metabolism*;
Child;
Interleukin-10/blood*;
Macrophages/metabolism*;
Tumor Necrosis Factor-alpha/blood*;
Interleukin-6/blood*;
Male;
Female;
Transforming Growth Factor beta/blood*;
Receptor, Macrophage Colony-Stimulating Factor/metabolism*;
Clinical Relevance
- From:
Journal of Experimental Hematology
2025;33(4):1131-1137
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of CSF-1 and CSF-1R in the peripheral blood of children with immune thrombocytopenia (ITP) and its clinical significance.
METHODS:Forty-four children with ITP treated in our hospital from February 2023 to January 2024 were selected as the observation group, and 40 healthy children were selected as the control group during the same period, and relevant clinical data were collected. Peripheral blood mononuclear cells (PBMC) of children with ITP and healthy children were separated, and the plasma levels of M1 macrophage-associated cytokines (TNF-α, IL-6), M2 macrophage-associated cytokines (IL-10, TGF-β), and CSF-1 were detected by ELISA in the children of both groups. The mRNA levels of M1 macrophage surface markers (CD86, iNOS), M2 macrophage surface markers (CD206, Arg-1) and CSF-1R were detected by RT-PCR in PBMC of children in both groups. Western blot was used to detect the expression of CSF-1R protein in PBMC of the two groups of children. The correlation between platelet count and CSF-1R mRNA expression in PBMC, TNF-α, IL-6, IL-10, TGF-β and CSF-1 in plasma was analyzed.
RESULTS:Compared with the control group, the levels of IL-10, TGF-β, CSF-1 and platelet count in plasma of children with ITP were significantly decreased (P < 0.01), and the levels of TNF-α and IL-6 were significantly increased (P < 0.01); the mRNA levels of the M1 macrophage surface markers (CD86, iNOS) in PBMC of children with ITP were significantly increased (P < 0.05), mRNA levels of M2 macrophage surface marker CD206 in PBMC of children with ITP were decreased compared with controls but the difference was not statistically significant ( P >0.05), mRNA levels of Arg-1 were decreased, the difference was statistically significant (P < 0.05). The mRNA and protein levels of CSF-1R in PBMC of ITP children were higher than that in controls. CSF-1R expression in PBMC of ITP was positively correlated with platelet count, IL-10, CSF-1 were positively correlated (r =0.822,0.481,0.405).
CONCLUSION:CSF-1 is significantly reduced in the plasma of ITP, and CSF-1R mRNA and protein expression is significantly elevated in PBMC of ITP, which are involved in the regulation of macrophage M1/M2 imbalance, and could serve as a potential therapeutic target for ITP.
