Effects of Oridonin on Platelet Function and Related Mechanisms
10.19746/j.cnki.issn1009-2137.2025.04.026
- VernacularTitle:冬凌草甲素对血小板功能的影响及相关作用机制研究
- Author:
Yu LI
1
;
Rong YAN
1
;
Meng-Nan YANG
1
;
Kang-Xi ZHOU
1
;
Ke-Sheng DAI
1
Author Information
1. 苏州大学苏州医学院,江苏省血液研究所,苏州大学第一附属医院,卫健委血栓与止血重点实验室,放射医学与防护国家重点实验室,血液学协同创新中心,江苏苏州 215006
- Publication Type:Journal Article
- Keywords:
oridonin;
platelet;
thrombosis;
activation
- From:
Journal of Experimental Hematology
2025;33(4):1104-1112
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of oridonin on platelet function and related mechanisms.Methods:Washed platelets from healthy adults and mice were incubated with different concentrations of oridonin(2.5,5 and 10μmol/L)in vitro.The surface expression level of P-selectin and the activation of integrin αⅡbβ3 in platelets were detected by flow cytometry,and the aggregation ability of platelets under the stimulation by various agonists was detected by light transmission aggregometry.The expression of P-AKT(Ser473)was detected by protein immunoblotting.Arterial thrombosis model was established in mice with mesenteric injury induced by ferric chloride,and tail hemorrhage model was established by cutting off the tail of mice.The effect of intraperitoneal injection of oridonin(10 mg/kg)on thrombosis and haemostasis was tested.Results:Oridonin inhibited platelet P-selectin expression and integrin αⅡbβ3 activation.In the presence of different stimulants,oridonin inhibited platelet aggregation in a concentration-dependent manner.The phosphorylation level of AKT Ser473 was reduced in the groups treated with different concentrations of oridonin.Oridonin significantly prolonged the time of mesenteric artery thrombosis in mice,but did not affect the tail bleeding time.Conclusion:Oridonin inhibits platelet activation,aggregation,and thrombosis by inhibiting AKT phosphorylation,and may be used as a potential antiplatelet drug.
