Effects of Oridonin on Platelet Function and Related Mechanisms.
10.19746/j.cnki.issn.1009-2137.2025.04.026
- Author:
Yu LI
1
;
Rong YAN
1
;
Meng-Nan YANG
1
;
Kang-Xi ZHOU
1
;
Ke-Sheng DAI
1
Author Information
1. Suzhou Medical College, Soochow University, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, State Key Laboratory of Radiation Medicine and Protection, Collaborative Innovation Center of Hematology, Suzhou 215006, Jiangsu Province, China.
- Publication Type:Journal Article
- Keywords:
oridonin;
platelet;
thrombosis;
activation
- MeSH:
Diterpenes, Kaurane/pharmacology*;
Animals;
Mice;
Blood Platelets/drug effects*;
Platelet Aggregation/drug effects*;
P-Selectin/metabolism*;
Thrombosis;
Platelet Glycoprotein GPIIb-IIIa Complex/metabolism*;
Proto-Oncogene Proteins c-akt/metabolism*;
Humans;
Phosphorylation;
Platelet Activation/drug effects*
- From:
Journal of Experimental Hematology
2025;33(4):1104-1112
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of oridonin on platelet function and related mechanisms.
METHODS:Washed platelets from healthy adults and mice were incubated with different concentrations of oridonin (2.5, 5 and 10 μmol/L) in vitro . The surface expression level of P-selectin and the activation of integrin αIIbβ3 in platelets were detected by flow cytometry, and the aggregation ability of platelets under the stimulation by various agonists was detected by light transmission aggregometry. The expression of P-AKT (Ser473) was detected by protein immunoblotting. Arterial thrombosis model was established in mice with mesenteric injury induced by ferric chloride, and tail hemorrhage model was established by cutting off the tail of mice. The effect of intraperitoneal injection of oridonin (10 mg/kg) on thrombosis and haemostasis was tested.
RESULTS:Oridonin inhibited platelet P-selectin expression and integrin αIIbβ3 activation. In the presence of different stimulants, oridonin inhibited platelet aggregation in a concentration-dependent manner. The phosphorylation level of AKT Ser473 was reduced in the groups treated with different concentrations of oridonin. Oridonin significantly prolonged the time of mesenteric artery thrombosis in mice, but did not affect the tail bleeding time.
CONCLUSION:Oridonin inhibits platelet activation, aggregation, and thrombosis by inhibiting AKT phosphorylation, and may be used as a potential antiplatelet drug.
