Effects of Bortezomib Combined with Polyphyllin Ⅶ on Proliferation, Apoptosis and Oxidative Stress of Myeloma Cells.
10.19746/j.cnki.issn.1009-2137.2025.03.026
- Author:
Ou-Xiao JI
1
;
Yao FU
2
;
Yu-Qing SUN
1
;
Li-Juan WANG
2
Author Information
1. Training Base of Linyi People's Hospital, Jinzhou Medical University,Linyi 276000, Shandong Province, China.
2. Central Laboratory of Linyi People's Hospital (Linyi Key Laboratory of Tumor Biology, Cultivation and Construction Site of Key Laboratory of Tumor Translational Medicine, Xuzhou Medical University).
- Publication Type:Journal Article
- Keywords:
ARH-77 myeloma cells;
polyphyllin Ⅶ;
bortezomib;
apoptosis;
oxidative stress
- MeSH:
Apoptosis/drug effects*;
Bortezomib;
Humans;
Cell Proliferation/drug effects*;
Oxidative Stress/drug effects*;
Multiple Myeloma/metabolism*;
Cell Line, Tumor;
Saponins/pharmacology*
- From:
Journal of Experimental Hematology
2025;33(3):802-809
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of bortezomib (BTZ) combined with polyphyllin Ⅶ (PP7) on proliferation, apoptosis and oxidative stress of myeloma cell line ARH-77.
METHODS:MTT assay was used to detect the inhibitory effects of different concentrations of BTZ, PP7 monotherapy, and their combination on the proliferation of ARH-77 cells. In subsequent experiments, the cells were divided into 4 groups: control group (no drug added), BTZ (15 nmol/L) group, PP7 (1.5 μmol/L) group and BTZ(15 nmol/L)+PP7 (1.5 μmol/L) group. The effects of the two drugs on the morphology of ARH-77 cells were observed. Flow cytometry was used to detect the apoptosis rate of the cells in each group. Calcein-AM/PI double staining kit was used to observe the status of the cells and the cell viability were evaluated. The expression of apoptosis-related proteins were detected by Western blot. DCFH-DA fluorescent probe was used to detect the levels of reactive oxygen species (ROS).
RESULTS:Both BTZ and PP7 monotherapy, as well as their combination, could inhibit the growth of ARH-77 cells in a dose-dependent manner (rBTZ=-0.9717, rPP7=-0.9941, rBTZ+PP7=-0.9951), and the combination of BTZ and PP7 exhibited a synergistic effect within a certain concentration range. Compared with the BTZ group and PP7 group, the apoptosis rate of the BTZ+PP7 group was significantly increased (P < 0.01), the expressions of pro-apoptotic proteins Bax, Smac and P53 were significantly upregulated (P < 0.05), the expression of anti-apoptotic protein Bcl-2 was significantly downregulated (P < 0.01), and the ratio of Bax/Bcl-2 was significantly increased (P < 0.01). Compared with the control group, the level of ROS in the BTZ, PP7 monotherapy group and BTZ+PP7 group were significantly increased (P < 0.05).
CONCLUSION:BTZ combined with PP7 can inhibit the proliferation and induce apoptosis of ARH-77 cells, and increase the level of intracellular ROS.
