The Effect of Histone Deacetylase on the Pathogenesis of Burkitt Lymphoma.
10.19746/j.cnki.issn.1009-2137.2025.03.025
- Author:
Chun-Tuan LI
1
;
Bing-Bing LI
2
;
Dan WENG
3
;
Wan-Lin YANG
3
;
Shao-Xiong WANG
1
;
Yan ZHENG
1
;
Dan WANG
3
;
Xiong-Peng ZHU
1
Author Information
1. Department of Hematology， The First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou 362200, Fujian Province, China.
2. Department of Respiratory and Critical Care Medicine, The Hospital Affiliated to Putian University, Quanzhou 362200, Fujian Province, China.
3. School of Clinical Medicine, Fujian Medical University, Quanzhou 362200, Fujian Province, China.
- Publication Type:Journal Article
- Keywords:
histone deacetylase;
Burkitt lymphoma;
PI3K/AKT/mTOR signaling pathway;
proliferation
- MeSH:
Humans;
Burkitt Lymphoma/pathology*;
Apoptosis;
Cell Proliferation;
Signal Transduction;
Proto-Oncogene Proteins c-akt/metabolism*;
Phosphatidylinositol 3-Kinases/metabolism*;
Cell Line, Tumor;
Histone Deacetylases/metabolism*;
TOR Serine-Threonine Kinases/metabolism*;
Histone Deacetylase Inhibitors/pharmacology*;
Phosphorylation
- From:
Journal of Experimental Hematology
2025;33(3):796-801
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of histone deacetylase (HDAC) levels on the proliferation and apoptosis of Burkitt lymphoma cells, and the changes in related signaling molecules in the PI3K/AKT/mTOR signaling pathway, so as to explore the pathogenesis of Burkitt lymphoma.
METHODS:HDAC levels in Burkitt lymphoma were detected by RT-PCR and Western blot. CA46 and RAJI cells were treated with the HDAC selective inhibitor VPA. CCK8 assay was used to detect the proliferation ability of cells. Western Blot was used to measure the expression of apoptosis-related proteins, PI3K/AKT/mTOR signaling pathway proteins and their phosphorylation levels.
RESULTS:The expression levels of classⅠ HDAC in Burkitt lymphoma were higher than those in normal cells, and the HDAC1 inhibitor VPA could inhibit the proliferation of CA46 and RAJI cells. VPA decreased HDAC expression in CA46 and RAJI cells, inhibited the phosphorylation of PI3K/AKT/mTOR pathway molecules AKT and p70S6K, increased the expression of apoptotic proteins Cleaved Caspase-3, Cleaved Caspase-8, Cleaved Caspase-9 and Bax, and decreased the expression of anti-apoptotic proteins Bcl-2 and PARP.
CONCLUSION:Inhibition of HDAC activity can Attenuate the proliferation of Burkitt lymphoma cells and induce apoptosis by inhibiting the PI3K/AKT/mTOR signaling pathway activity.
