Clinical and Laboratory Characteristics of Acute Myeloid Leukemia, Myelodysplasia-Related.
10.19746/j.cnki.issn.1009-2137.2025.03.007
- Author:
Wei-Bin LI
1
;
Lan YANG
1
;
Shao-Jie CHENG
1
;
Ya CHEN
1
;
Yan JIANG
1
Author Information
1. Department of Clinical Laboratory Medicine, The 900th Hospital of Joint Logistics Support Force, PLA ,Fuzhou 350025, Fujian Province, China.
- Publication Type:Journal Article
- Keywords:
acute myeloid leukemia, myelodysplasia-related;
bone marrow cytology;
flow cytometry;
chromosome karyotype analysis;
cytogenetics
- MeSH:
Humans;
Leukemia, Myeloid, Acute/diagnosis*;
Myelodysplastic Syndromes;
Flow Cytometry;
Karyotyping;
Male;
Middle Aged;
Mutation
- From:
Journal of Experimental Hematology
2025;33(3):666-671
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To understand clinical and laboratory characteristics of acute myeloid leukemia, myelodysplasia-related (AML-MR).
METHODS:Blood sample of one patient with AML-MR admitted to our hospital in September 2021 was collected and synthetically analyzed by using techniques including complete blood cell count, peripheral blood and bone marrow cell morphology, bone marrow pathology and immunohistochemistry, hematology examination, flow cytometry (FCM), chromosome karyotype analysis and molecular pathology. The clinical and laboratory characteristics of AML-MR were analyzed and summarized according to the World Health Organization (WHO) standards.
RESULTS:The patient showed pancytopenia and increased proportion of blasts in smear of peripheral blood cells. Bone marrow cytology and pathological examination showed significant proliferation of hematopoietic cells. Pathological immunohistochemistry showed increased expression of CD61, CD34, and CD117, while MPO, CD13, and CD33 were positive. FCM showed that abnormal myeloid progenitor cells accounted for approximately 18.61% of the total number of nuclear cells, with expression of CD34, CD13, CD117, HLA-DR, and CD33 (small amount). Additionally, 36.34% of the cells were primitive/immature red blood cells which expressed CD36, CD71, and CD117 (small amount). Chromosome karyotype analysis and molecular pathology detected three kinds of abnormalities including -5 and two kinds of TP53 related gene mutation, respectively.
CONCLUSION:AML-MR patient shows pancytopenia and increased proportion of blasts in smear of peripheral blood cells. Bone marrow cytology and pathological examination show significant proliferation of hematopoietic cells. FCM can detect myeloid progenitor cells and primitive/immature red blood cells, while chromosome karyotype analysis can detect three abnormal karyotypes.
