Genotyping and Transfusion Strategy for Pregnant Patients with ABO Blood Typing Difficulties.
10.19746/j.cnki.issn.1009-2137.2025.02.034
- Author:
Chen-Chen FENG
1
;
Qing CHEN
2
;
Xiao WEI
3
;
Li-Li SHI
1
;
Ruo-Yang ZHANG
1
;
Fang ZHAO
1
;
Jian-Yu XIAO
1
Author Information
1. Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China.
2. Department of Blood Transfusion, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu Province, China.
3. Key Laboratory of Yingbing Diagnosis and Treatment, Nanjing University of Chinese Medicine Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing 210028, Jiangsu Province, China.
- Publication Type:Journal Article
- Keywords:
ABO subtype;
genotyping;
safe blood transfusion;
prenatal screening
- MeSH:
Humans;
Female;
Pregnancy;
ABO Blood-Group System/genetics*;
Blood Grouping and Crossmatching;
Blood Transfusion;
Genotype;
Phenotype
- From:
Journal of Experimental Hematology
2025;33(2):538-545
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To identify the blood type of specimens from pregnant patients with difficult-to-type ABO status, and to guide clinical safe blood transfusion.
METHODS:The specimens from 36 pregnant patients with suspicious ABO blood group were collected. These specimens were submitted by clinical institutions from various regions to our center's genetic testing platform from January 2021 to December 2022. The blood group phenotypes and genotypes of these specimens were identified by serological method and genetic sequencing.
RESULTS:A total of 20 ABO subtypes were detected in the 36 samples, including 10 cases of BA/O, 3 cases of cisAB/O, 2 cases of A/Bw, 1 case of A2/B, 1 case of Aw/B, 1 case of BA/B, 1 case of BA/A, and 1 case of Bw/O. Additionally, 4 cases were identified as para-Bombay blood type, and no specific variations associated with abnormal phenotypes were found in the remaining 12 cases.
CONCLUSION:ABO subtypes interfere with ABO blood group identification in pregnant patients, and pregnancy status also affects blood group phenotype. Accurate determination of blood group genotype by genetic sequencing technology can guide clinical blood transfusion for pregnant patients, and ensure maternal and infant safety.
