Expression and Function of miR-144 in β-Thalassemia.
10.19746/j.cnki.issn.1009-2137.2025.02.027
- Author:
Lan YANG
1
;
Ling LING
1
;
Fan YANG
1
;
Lei YANG
1
;
Zhi-Chen DAI
1
;
Duo-Nan YU
1
Author Information
1. Yangzhou University Medical College/Jiangsu Key Laboratory of Experimental & Translational Noncoding RNA Research, Yangzhou 225009, Jiangsu Province, China.
- Publication Type:Journal Article
- Keywords:
miR-144;
erythroid differentiation;
β-thalassemia
- MeSH:
MicroRNAs/metabolism*;
beta-Thalassemia/genetics*;
Animals;
Mice;
Humans;
Cell Differentiation;
Hepatocytes
- From:
Journal of Experimental Hematology
2025;33(2):491-497
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the expression and function of microRNA-144 (miR-144) in β-thalassemia (β-thal).
METHODS:The expression of miR-144 during the differentiation of murine erythroleukemia (MEL) cells and mouse embryonic liver-derived erythroid precursor cells was analyzed by real-time fluorescence quantitative PCR (qRT-PCR); The expression levels of miR-144 in peripheral blood and day-14.5 embryonic hepatocytes of wild-type (WT) and β-thal mice, as well as the expression levels of miR-144 in peripheral blood of β-thal patients, was also measured by qRT-PCR. The proportion of Ter119 and CD71 double positive cells in peripheral blood of mild and severe β-thal mice was analyzed by flow cytometry, and the expression levels of miR-144 in the peripheral blood of mild and severe β-thal mice and patients were compared; Bone marrow nucleated erythrocytes from WT mice and β-thal mice were sorted and the expression levels of miR-144 potential target genes were analyzed by gene chip.
RESULTS:The expression levels of miR-144 were gradually increased during the directed differentiation of mouse MEL cells and embryonic hepatocytes to the erythroid lineage (r MEL=0.97, r embryonic hepatocytes=0.86); Compared with WT mice, the expression levels of miR-144 in peripheral blood and 14.5-day embryonic hepatocytes of β-thal mice were significantly increased (P < 0.05); Compared with healthy controls, the patients with β-thal showed an increased expression levels of miR-144 in peripheral blood (P < 0.05). Compared with mice and humans with mild β-thal, the expression levels of miR-144 in peripheral blood of those with severe β-thal were significantly increased (P < 0.05). The expressions of potential target genes of miR-144 in nucleated erythroid cells of the β-thal mice were significantly reduced compared to the WT group.
CONCLUSION:The expression level of miR-144 gradually increases in erythroid development, and compared with mild β-thal patients, the expression level of miR-144 in the peripheral blood is higher in severe β-thal patients. MiR-144 is expected to be an auxiliary diagnostic indicator for β-thal in clinical practice.
