Effect of TBL1XR1 Mutation on Cell Biological Characteristics of Diffuse Large B-Cell Lymphoma.
10.19746/j.cnki.issn.1009-2137.2025.02.016
- Author:
Hong-Ming FAN
1
;
Le-Min HONG
1
;
Chun-Qun HUANG
1
;
Jin-Feng LU
1
;
Hong-Hui XU
1
;
Jie CHEN
1
;
Hong-Ming HUANG
1
;
Xin-Feng WANG
1
;
Dan GUO
1
Author Information
1. Department of Hematology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China.
- Publication Type:Journal Article
- Keywords:
diffuse large B-cell lymphoma
- MeSH:
Humans;
Lymphoma, Large B-Cell, Diffuse/pathology*;
Cell Proliferation;
Mutation;
Receptors, Cytoplasmic and Nuclear/genetics*;
Apoptosis;
Cell Line, Tumor;
Epithelial-Mesenchymal Transition;
Cell Movement;
Repressor Proteins/genetics*;
Nuclear Proteins/genetics*;
Cadherins/metabolism*
- From:
Journal of Experimental Hematology
2025;33(2):423-430
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of TBL1XR1 mutation on cell biological characteristics of diffuse large B-cell lymphoma (DLBCL).
METHODS:The TBL1XR1 overexpression vector was constructed and DNA sequencing was performed to determine the mutation status. The effect of TBL1XR1 mutation on apoptosis of DLBCL cell line was detected by flow cytometry and TUNEL fluorescence assay; CCK-8 assay was used to detect the effect of TBL1XR1 mutation on cell proliferation; Transwell assay was used to detect the effect of TBL1XR1 mutation on cell migration and invasion; Western blot was used to detect the effect of TBL1XR1 mutation on the expression level of epithelial-mesenchymal transition (EMT) related proteins.
RESULTS:The TBL1XR1 overexpression plasmid was successfully constructed. The in vitro experimental results showed that TBL1XR1 mutation had no significant effect on apoptosis of DLBCL cells. Compared with the control group, TBL1XR1 mutation enhanced cell proliferation, migration and invasion of DLBCL cells. TBL1XR1 gene mutation significantly increased the expression of N-cadherin protein, while the expression of E-cadherin protein decreased.
CONCLUSION:TBL1XR1 mutation plays a role in promoting tumor cell proliferation, migration and invasion in DLBCL. TBL1XR1 could be considered as a potential target for DLBCL therapy in future research.
