Metabolic Characteristics of 18F-FDG in Different Types of Myeloid Leukemia Cells and Tumor-Bearing Nude Mice.
10.19746/j.cnki.issn.1009-2137.2025.02.003
- Author:
Xi CHEN
1
;
Qin YAN
1
;
Xiang QIN
1
;
Li ZHANG
1
;
Yue FENG
2
;
Qian CHEN
1
;
Si-Li LONG
1
;
Wen-Jun LIU
1
Author Information
1. Department of Pediatrics, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.
2. Department of Nuclear Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.
- Publication Type:Journal Article
- Keywords:
leukemia
- MeSH:
Animals;
Fluorodeoxyglucose F18/metabolism*;
Mice, Nude;
Mice;
Humans;
Leukemia, Myeloid/diagnostic imaging*;
HL-60 Cells;
K562 Cells;
Cell Line, Tumor;
U937 Cells
- From:
Journal of Experimental Hematology
2025;33(2):325-330
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the metabolic characteristics of 18F-fluorodeoxyglucose (18F-FDG) in myeloid leukemia by in vitro culture of myeloid leukemia cells and construction of tumor-bearing nude mouse model.
METHODS:U937, THP-1, HL60 and K562 cells were cultured in vitro. The cells in logarithmic growth phase (l×10 5 cells/well) were added with 18F-FDG, and the uptake rate of 18F-FDG was measured at 15, 30, 60 and 120 min after addation, respectively. The four kinds of cells were inoculated subcutaneously into the hind limbs of nude mice to establish a tumor-bearing nude mouse model. When the tumor size was about 500 mm3, 18F-FDG was injected through the tail vein of the mice, and positron emission tomography/computed tomography was performed at 60 min after injection. The morphology of tumor-bearing cells was observed by hematoxylin-eosin (HE) staining in serial pathological sections.
RESULTS:After co-incubation with 18F-FDG, the 18F-FDG uptake rates of U937 cells were significantly higher than THP-1, HL60 and K562 cells at 4 time points (all P <0.05), and THP-1 cells were higher than K562 cells (all P <0.05). The uptake rate of 18F-FDG by leukemia cells was rapid in the first 60 min, then tended to be stable. Pathological analysis showed that subcutaneous inoculation of U937, THP-1, HL60 and K562 cells could successfully establish tumor-bearing nude mouse models of myeloid leukemia. The 18F-FDG uptake value in U937 tumor-bearing nude mice was significantly higher than THP-1, HL60 and K562 tumor-bearing nude mice (all P <0.01). The 18F-FDG uptake values in THP-1 and HL60 tumor-bearing nude mice were significantly higher than that in K562 tumor-bearing nude mice (both P <0.01).
CONCLUSION:The tumor-bearing nude mouse model of myeloid leukemia can be successfully constructed by subcutaneous inoculation. The 18F-FDG uptake rate of acute myeloid leukemia (AML) cells is higher in cells cultured in vitro and tumor-bearing nude mouse model. 18F-FDG may have better clinical application value for AML.
