Effect of Iron Overload on Expression of PD-1 on Surface of T Lymphocyte in Mice
10.19746/j.cnki.issn1009-2137.2025.01.039
- VernacularTitle:铁过载对小鼠T细胞表面PD-1表达的影响
- Author:
Yu-Mei LIU
1
;
Hua-Quan WANG
1
;
Zong-Hong SHAO
1
Author Information
1. 天津医科大学总医院血液科,天津 300052
- Publication Type:Journal Article
- Keywords:
iron overload;
T lymphocyte;
programmed death-1;
reactive oxygen species
- From:
Journal of Experimental Hematology
2025;33(1):262-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of iron overload on the expression of programmed death-1(PD-1)on the surface of T lymphocyte in mice,in order to analyze the mechanism of iron overload inhibiting T cell function.Methods:Flow cytometry was used to detect the labile iron pool(LIP),reactive oxygen species(ROS),and the expression of PD-1 in peripheral blood T cells in mice with iron overload.Results:The mean fluorescence intensity of calcein in T cells of mice in iron overload group was 2 492±311.1,which was significantly lower than 3 136±537.3 in the control group(P＜0.01),suggesting that increased LIP in iron overload group.Compared with the control group,the ratio of CD4/CD8 of peripheral blood T cells was normal or increased in iron overload group.The level of ROS in T cells was 2 452±393.3 in iron overload group,which was significantly increased compared to 1 874±121.8 in the control group(P＜0.001).The expression of PD-1 on the surface of T cells was significantly increased.The percentage of PD-1+cells in CD8+T cells was(12.97±6.92)％and(6.18±2.95)％in iron overload group and control group,respectively(P＜0.05),and that in CD8-T cells was(33.55±15.69)％and(12.51±4.11)％(P＜0.001).Conclusion:The expression of PD-1 on peripheral blood T cells in mice with iron overload is significantly increased,which may be involved in inhibiting T cell effector function.
