Effect of Temperature Cycle Preservation on Platelet Aggregation Rate and Routine Parameters.
10.19746/j.cnki.issn.1009-2137.2025.01.035
- Author:
Ju-Ling LIANG
1
;
Zhi-Hao DENG
1
;
Chuang-Jin ZHUO
1
;
Lu HUANG
1
;
Jing XU
1
;
Wei-Jian WU
1
Author Information
1. Foshan Central Blood Station, Foshan 528000, Guangdong Province, China.
- Publication Type:Journal Article
- Keywords:
temperature cycling preservation;
platelet;
platelet aggregation rate;
cold storage
- MeSH:
Humans;
Platelet Aggregation;
Blood Preservation/methods*;
Temperature;
Blood Platelets;
Platelet Count;
Mean Platelet Volume;
Cryopreservation/methods*;
Cold Temperature
- From:
Journal of Experimental Hematology
2025;33(1):236-240
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare and analyze the changes of aggregation rate and routine parameters of platelets stored in temperature cycle, cold storage at 4 ℃ and oscillating storage at 22 ℃, so as to provide more experimental data for platelet preservation methods.
METHODS:Blood samples were collected at 5 time points on the 1st, 2nd, 3rd, 4th and 6th day after platelet cycling preservation at temperature, cold storage at 4 ℃, and oscillating storage at 22 ℃. Platelet maximum aggregation rate (MAR) and routine parameters including platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW) and platelet-larger cell ratio (P-LCR) were detected.
RESULTS:The platelet MAR of three groups showed a significant decrease trend with the preservation time, the fastest decrease was in the 22 ℃ group, the slowest was in the 4 ℃ group, and the temperature cycle group was between the two groups. On the 3rd day of preservation, the platelet MAR in 4 ℃ group was still in the normal range (MAR>60%), while in temperature cycle group was about 50%, and in 22 ℃ group was the lowest. On the 4th day of preservation, platelet MAR in all the three groups was lower than 50%, and that in temperature cycle group was significantly lower than in 4 ℃ group but higher than in 22 ℃ group (both P < 0.05). On the 6th day of preservation, platelet MAR in the temperature cycle group was significantly lower than that in the 4 ℃ group ( P <0.05), but there was no statistically significant difference compared to 22 ℃ group (P >0.05). PLT values in the three groups were all significantly decreased with the preservation time extension, and were significantly lower than those in the early stage of preservation within 6 days (all P < 0.05). PDW in temperature cycle group had no significant change within 6 days of preservation, but MPV and P-LCR were significantly increased. MPV, PDW and P-LCR all decreased significantly in 4 ℃ group within 6 days of preservation but increased in 22 ℃ group. Under the same storage days, PLT value of temperature cycle group had no significant difference with that of 4 ℃ group and 22 ℃ group, while MPV, PDW and P-LCR values were significantly higher than 4 ℃ group but lower than 22 ℃ group (all P < 0.05).
CONCLUSION:The aggregation function and routine parameters changes of temperature circulating preserved platelets are between 4 and 22 ℃.
