Serological and Molecular Biological Characteristics of cisAB Blood Group and Transfusion Strategies
10.19746/j.cnki.issn1009-2137.2025.01.030
- VernacularTitle:cisAB血型血清学和分子生物学特点及输血策略
- Author:
Si-Meng WU
1
;
Qiao-Ni YANG
1
;
Wa GAO
1
;
Xiao-Shuai LI
1
;
Qiu-Shi WANG
1
Author Information
1. 中国医科大学附属盛京医院输血科,辽宁沈阳 110004
- Publication Type:Journal Article
- Keywords:
cisAB;
blood group;
serology;
molecular biology;
transfusion strategy
- From:
Journal of Experimental Hematology
2025;33(1):206-210
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the serological and molecular biological characteristics of 5 patients with cisAB blood group,and to explore the safe transfusion strategy.Methods:Serological identification of the samples'blood group was performed using anti-A,anti-B,anti-D,anti-A1,anti-H typing reagents and ABO reagent erythrocytes.Molecular biological identification of the samples'blood group was performed using PCR-SSP or gene sequencing.Results:The serological identification results of blood group in 5 patients all showed inconsistent forward and reverse typing,presenting as A2B3 or A2Bw.ABO gene sequencing of samples 1,2 and 3 showed 261delG in exon 6 and 467C＞T,803G＞C in exon 7.The genotypes of samples 1,2 and 3 were determined to be cisAB/O.PCR-SSP genotyping was performed on sample 4 and 5,and the results were both cisAB/O.Conclusion:Patients with cisAB alleles have inconsistent serological manifestations,and genetic testing is necessary to ensure the safety and effectiveness of blood transfusion.
