Serological and Molecular Biological Characteristics of cisAB Blood Group and Transfusion Strategies.
10.19746/j.cnki.issn.1009-2137.2025.01.030
- Author:
Si-Meng WU
1
;
Qiao-Ni YANG
1
;
Wa GAO
1
;
Xiao-Shuai LI
1
;
Qiu-Shi WANG
1
Author Information
1. Department of Blood Transfusion，Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.
- Publication Type:Journal Article
- MeSH:
Humans;
ABO Blood-Group System/genetics*;
Blood Transfusion;
Blood Grouping and Crossmatching;
Genotype;
Blood Group Antigens/genetics*;
Alleles;
Male;
Female
- From:
Journal of Experimental Hematology
2025;33(1):206-210
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the serological and molecular biological characteristics of 5 patients with cis AB blood group, and to explore the safe transfusion strategy.
METHODS:Serological identification of the samples' blood group was performed using anti-A, anti-B, anti-D, anti-A1, anti-H typing reagents and ABO reagent erythrocytes. Molecular biological identification of the samples' blood group was performed using PCR-SSP or gene sequencing.
RESULTS:The serological identification results of blood group in 5 patients all showed inconsistent forward and reverse typing, presenting as A2B3 or A2Bw. ABO gene sequencing of samples 1, 2 and 3 showed 261delG in exon 6 and 467C>T, 803G>C in exon 7. The genotypes of samples 1, 2 and 3 were determined to be cisAB/O . PCR-SSP genotyping was performed on sample 4 and 5，and the results were both cisAB/O .
CONCLUSION:Patients with cisAB alleles have inconsistent serological manifestations, and genetic testing is necessary to ensure the safety and effectiveness of blood transfusion.
