Influencing Factors of FeCl3 Induced Mouse Carotid Artery Thrombosis Model.
10.19746/j.cnki.issn.1009-2137.2025.01.028
- Author:
Jia-Hao DU
1
;
Li-Li ZHAO
1
;
Biao YANG
1
;
Ke-Sheng DAI
1
Author Information
1. Suzhou Medical College of Soochow University, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, State Key Laboratory of Radiation Medicine and Protection, Collaborative Innovation Center of Hematology, Suzhou 215006, Jiangsu Province, China.
- Publication Type:Journal Article
- Keywords:
platelet;
carotid artery;
ferric chloride
- MeSH:
Animals;
Mice;
Ferric Compounds/adverse effects*;
Chlorides;
Disease Models, Animal;
Carotid Artery Thrombosis/chemically induced*;
Male
- From:
Journal of Experimental Hematology
2025;33(1):193-197
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the factors affecting ferric chloride (FeCl3) - induced carotid artery thrombosis model experiment.
METHODS:After the common carotid artery was damaged by FeCl3, the injured vessels were dissected for fixation, embedding, frozen section, and then processed HE staining. The carotid thrombus area ratio was calculated. We examined the effect of FeCl3 concentration (5%, 10% and 15%), reaction time (2, 4 and 6 min), and recipient mouse age (4-5, 6-8 and 10 weeks) on the formation and stability of arterial thrombosis model. The model was injected through the post-glomus venous plexus of mouse eyeball with 0.075 μg/g and 0.1 μg/g R300 to verify the accuracy of the FeCl3-induced model on thrombus formation by adjusting the platelet number.
RESULTS:HE staining showed that thrombus formation induced by 10% and 15% FeCl3 was more stable, dense and larger than 5% FeCl3-induced thrombosis. 10% FeCl3 induced the formation of dense and large thrombosis after 4 and 6 minutes of vascular endothelium injury, while the thrombosis induced for 2 minutes were looser and smaller in area. Mouse age can not affect thrombus formation and stability, because there were no significant differences in the formation of dense thrombus and thrombus area induced by 10% FeCl3 among three different age groups of mice.
CONCLUSION:Many factors affect the formation and stability of arterial thrombosis model induced by FeCl3. This optimal experimental conditions for construction of a stable carotid artery thrombosis model are 10% FeCl3, 4 minutes for injury, and 6-8 week old mice.
