Scleromitrion diffusum reverses epithelial-mesenchymal transi-tion of gastric mucosa in rats with gastric precancerous lesions.
10.3724/zdxbyxb-2024-0536
- Author:
Luping MA
1
;
Xin ZUO
2
;
Weikai ZHU
2
;
Jiyan LI
3
;
Yanyan ZHAO
2
;
Jingyuan ZHANG
2
;
Hui SHEN
4
Author Information
1. Department of Traditional Chinese Medicine, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China. maluping@firsthosp-dmu.com.
2. Department of Traditional Chinese Medicine, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China.
3. Out-Patient Department, Dalian Hospital of Traditional Chinese Medicine, Dalian 116013, Liaoning Province, China.
4. Department of Traditional Chinese Medicine, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China. shenhui@firsthosp-dmu.com.
- Publication Type:Journal Article
- Keywords:
Epithelial-mesenchymal transition;
Gastric precancerous lesion;
SD rats;
Scleromitrion diffusum
- MeSH:
Animals;
Rats;
Rats, Sprague-Dawley;
Epithelial-Mesenchymal Transition/drug effects*;
Precancerous Conditions/metabolism*;
Gastric Mucosa/metabolism*;
Stomach Neoplasms/drug therapy*;
Male;
Cadherins/metabolism*
- From:
Journal of Zhejiang University. Medical sciences
2025;54(3):342-349
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To investigate the effect of Scleromitrion diffusum on gastric mucosal epithelial-mesenchymal transition (EMT) in rats with gastric precancerous lesion.
METHODS:Fifty SD rats were randomly divided into blank control group (n=11), model control group (n=13), Scleromitrion diffusum (SD) group (n=13) and vitase group (n=13). Gastric precancerous lesion animal model was prepared by 1-methyl-3-nitro-1-nitrosoguanidine complex polyfactor method, and the drugs were administrated by gavage once a day for 6 weeks. The pathological changes of gastric mucosa were observed with hematoxylin and eosin staining, the expression of EMT marker proteins were detected with immunohistochemical staining and Western blotting.
RESULTS:Compared with the model control group, the gastric mucosal injury was significantly attenuated in the Scleromitrion diffusum group, the mucosal tissue structure gradually recovered, the saccular expansion area was reduced, and the inflammatory infiltration was ameliorated. The expression of epithelial cadherin was higher, and the expression of neural cadherin and vimentin in the Scleromitrion diffusum group were lower than those of model control group (all P<0.05).
CONCLUSIONS:Scleromitrion diffusum can ameliorate gastric mucosal injury in rats with gastric precancerous lesion by reversing the EMT.
