Determination of vitamin D3 content in cod liver oil using a column-switching technique.
10.3724/zdxbyxb-2024-0045
- Author:
Lyuye QI
1
;
Liyuan ZHANG
2
;
Qiaoyuan CHENG
2
;
Linqi YAN
2
;
Minghao ZHOU
3
Author Information
1. Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China. qilvye@zjyj.org.cn.
2. Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China.
3. Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China. zhouminghao@zjyj.org.cn.
- Publication Type:Journal Article
- Keywords:
Assay;
Cod liver oil;
Two-dimensional liquid chromatography;
Vitamin D 3
- MeSH:
Cholecalciferol/analysis*;
Cod Liver Oil/chemistry*;
Chromatography, Liquid/methods*;
Chromatography, High Pressure Liquid/methods*
- From:
Journal of Zhejiang University. Medical sciences
2024;53(6):779-784
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To develop a two-dimensional liquid chromatography method to determine the content of vitamin D3 in cod liver oil preparations.
METHODS:The samples were prepared by saponification and extraction, and the content of vitamin D3 was determined by two-dimensional liquid chromatography with dual-pump-single-valve switching dual detectors. The chromatographic column, capture device, and detection wavelength were optimized; the linearity, system suitability, recovery rate, repeatability and sample stability of the method were investigated, and further validated in actual sample determination.
RESULTS:A column switching two-dimensional chromatography method was developed. In the first chromatography dimension, an Agilent PoroShell SB-C8 (50 mm×4.6 mm, 2.7 μm) column was used with acetonitrile-water as the mobile phase with gradient elution at a flow rate of 1.0 mL/min and detection wavelength as 264 nm. An Agilent Zorbax SB-C18 (150 mm×3.0 mm,1.8 μm) column was used in the second chromatography dimension with acetonitrile as the mobile phase at a flow rate of 0.42 mL/min, and detection wavelength as 264 nm. In determining vitamin D3 content, there was a good linear relationship in the concentration range. The system suitability, recovery rate, repeatability and sample stability all met verification requirements.
CONCLUSIONS:The two-dimensional liquid chromatography method developed in this study is accurate, reproducible and simple, and can simultaneously separate pre-vitamin D3, trans-vitamin D3, vitamin D3, and tachysterol D3.
