Assessing the efficacy of a novel sperm-washing medium enriched with serotonin, L-carnitine, and coenzyme Q10: an observational cohort study.

Sinem DOGAN; Turgut AYDIN; Nadiye KOROGLU; Yasemin YILMAZER; Nazli ALBAYRAK; Fadime CETIN; Elnaz MOSHFEGHI; Ozge CELIK

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Assessing the efficacy of a novel sperm-washing medium enriched with serotonin, L-carnitine, and coenzyme Q10: an observational cohort study.

Author: Sinem DOGAN ¹ ; Turgut AYDIN ² ; Nadiye KOROGLU ² ; Yasemin YILMAZER ³ ; Nazli ALBAYRAK ² ; Fadime CETIN ⁴ ; Elnaz MOSHFEGHI ⁵ ; Ozge CELIK ¹
Author Information

1. Department of Molecular Biology and Genetics, Istanbul Kultur University, Istanbul 34158, Türkiye.
2. Department of Obstetrics and Gynecology, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul 34303, Türkiye.
3. Department of Molecular Biology and Genetics, Istanbul Sabahattin Zaim University, Istanbul 34303, Türkiye.
4. Department of Bioengineering, Istanbul Yildiz Technical University, Istanbul 34349, Türkiye.
5. Department of Molecular Biology and Genetics, Istanbul Yildiz Technical University, Istanbul 34349, Türkiye.
Publication Type:Observational Study
MeSH: Humans; Male; Serotonin; Carnitine/pharmacology*; Ubiquinone/pharmacology*; Sperm Motility/drug effects*; Adult; Spermatozoa/drug effects*; Cohort Studies; Reactive Oxygen Species/metabolism*; Culture Media; DNA Fragmentation/drug effects*; Apoptosis/drug effects*; DNA Damage/drug effects*
From: Asian Journal of Andrology 2024;26(6):635-639
CountryChina
Language:English
Abstract: This observational cohort study investigated the potential of a novel sperm-washing medium (SWM) enriched with serotonin (5-HT), L-carnitine (L-C), and coenzyme Q10 (CoQ10) to enhance sperm motility and reduce DNA damage. It compared this innovative medium (5-HT/L-C/CoQ10 SWM) with two widely used commercial media (SWM 1 and SWM 2). Ninety-eight volunteers from an infertility clinic provided semen samples, which were divided into three aliquots for analysis in different SWMs: group 1, SWM was composed of hydroxyethyl piperazineethanesulfonic acid (HEPES), sodium bicarbonate, human serum albumin (HSA), taurine, and gentamicin sulfate (SWM 1); group 2, SWM was composed of HEPES, sodium bicarbonate, and HSA (SWM 2); and group 3, SWM was composed of HEPES-buffered human tubal fluid supplemented with 5-HT, L-C, and CoQ10 (5-HT/L-C/CoQ10 SWM). Sperm motility was categorized as progressive, nonprogressive, or immotile. Apoptosis, reactive oxygen species (ROS) production, and DNA fragmentation were also assessed. There were no significant differences in total or progressive sperm motility among the groups. Spermatozoa in group 3 exhibited reduced apoptosis, necrosis, and ROS levels and increased viability. No significant differences were observed in the DNA fragmentation index among groups. The 5-HT/L-C/CoQ10 SWM reduced sperm oxidative stress and apoptosis compared with those of the two commercially available SWMs, suggesting that 5-HT/L-C/CoQ10 SWM could be useful for enhancing in vitro fertilization success rates.