Analyze of HLA class-I antibody and epitopes in patients with ineffective platelet transfusion in Shandong region.
- Author:
Jingru SHAO
1
,
2
Author Information
1. Blood Center of Shandong Provincial, HLA Laboratory, Jinan 250000, China. *Corresponding author, E-mail: shaojr0425@
2. com.
- Publication Type:Journal Article
- MeSH:
Humans;
Platelet Transfusion;
Male;
Female;
Middle Aged;
Epitopes/genetics*;
China;
Adult;
Histocompatibility Antigens Class I/genetics*;
Antibodies/blood*;
Aged;
Young Adult;
HLA-A Antigens/genetics*;
Adolescent;
HLA-B Antigens/genetics*
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(11):1013-1019
- CountryChina
- Language:Chinese
-
Abstract:
Objective Analyze the human leukocyte antigen(HLA) class-I antibody specificity and antigenic determinants in patients with ineffective platelet transfusion, to provide theoretical basis for the establishment and application of platelet donor bank in Shandong Province. Methods 69 patients with ineffective platelet transfusion, the patients specimens were subjected to specific detection of HLA class-I antibody, and the possible antigenic determinants were analyzed using HLA Matchmaker software, and the relative immunogenicity of the antigen was calculated. Results A total of 113 specific antibodies were detected in 69 patient specimens. Among which 33 were antibodies to the HLA-A loci, with the highest frequency of HLA-A*69:01 (54%), 54 were antibodies to the HLA-B loci, and the highest frequency of HLA-B*15:12 (64%); 25 antibodies against Cw loci with low platelet expression were detected, with HLA-C*17:01 having the highest frequency (38%). Using HLA Matchmaker software, a total of 221 HLA class I epitopes were detected, among which 163LG and 163L had the highest probability, reaching 59.4%. Among the HLA-A loci, the allele HLA-A*02:03 has the highest relative immunogenicity at 137.157, while the allele HLA-A*02:05 has the lowest relative immunogenicity at 0.1450. In the HLA-B locus, the relative immunogenicity of HLA-B*73:01 allele is the highest, reaching 229.885, while the relative immunogenicity of HLA-B*13:02 allele is the lowest, reaching 0.121. Conclusion This study obtained the distribution characteristics of HLA class-I antibodies in PTR patients in Shandong population, providing theoretical basis for precise platelet transfusion, improving transfusion efficiency, and establishing and applying platelet supply banks.
