Effect of different culture time on immunomembrane proteins of human monocyte-derived dendritic cells and their exosomes.
- Author:
Shumin LUO
1
;
Fang XU
1
;
Pengpeng LU
1
;
Yiyue WANG
1
;
Chuanyun LI
2
,
3
;
Weihua LI
4
Author Information
1. Beijing Institute of Infectious Diseases Integrated with Traditional Chinese and Western Medicine, Beijing Youan Hospital, Capital Medical University, Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China.
2. Department of Surgery, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China. *Corresponding authors, E-mail: lichuany0388@
3. com.
4. Beijing Institute of Infectious Diseases Integrated with Traditional Chinese and Western Medicine, Beijing Youan Hospital, Capital Medical University, Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China. *Corresponding authors, E-mail: liweihua@ccmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Humans;
Dendritic Cells/immunology*;
Exosomes/immunology*;
Monocytes/metabolism*;
Cells, Cultured;
Time Factors;
B7-1 Antigen/metabolism*;
Membrane Proteins/immunology*;
Cell Culture Techniques/methods*;
B7-2 Antigen/metabolism*;
Cell Differentiation;
CD11c Antigen/metabolism*;
Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology*
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(11):971-977
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate how culture duration affects the expression of immune membrane proteins in human monocyte-derived dendritic cells (DCs) and their exosomes (DEXs). Methods Human monocytes were induced with recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) to differentiate into DCs and were subsequently matured with tumor necrosis factor α(TNF-α). Exosomes were isolated by ultracentrifugation, and DEXs were identified by transmission electron microscopy and Amnis imaging flow cytometry, which were also used to quantify the expression of immune membrane proteins on DCs and DEXs. Results On the 10th day of culture, DCs displayed high surface expression of CD11c, CD80, CD86, major histocompatibility complex class I (MHC-I), and MHC-II. Expression peaked at day 18(CD11c: 78.66%±20.33%, CD80: 76.41%±10.02%, CD86: 96.43%±0.43%, MHC-I: 84.71%±2.96%, MHC-II: 80.01%±7.03%). After day 24, the overall expression showed a declining trend, with statistically significant differences observed for all markers except CD80 and MHC-II. By day 30, 80% of the DCs still expressed CD80, CD86, and MHC-II. The expression of immune membrane proteins on DEX surfaces also reached its peak on day 18, followed by an overall decline with prolonged culture time, with statistically significant differences observed for all markers except CD80. Correlation analysis revealed a significant positive relationship between the expression levels of immune membrane proteins on DC and DEX surfaces (CD11c: r=0.98; CD80: r=0.65; CD86: r=0.82; MHC-I: r=0.86; MHC-II: r=0.93). Conclusion Human monocyte-derived DCs in vitro express high expression of immune membrane proteins and maintain stable expression over a specific period. The exosomes secreted by these cells similarly demonstrate high surface expression of immune membrane proteins, with temporal trends aligned with those of the parent DCs.
