She-Ti-Zhi-Qiu decoction ameliorates allergic rhinitis in rats by regulating the gut microbiota and Th17/Treg balance.
- Author:
Chao LUO
1
;
Lisha LIU
2
;
Yajing HUANG
3
;
Shaobo LIU
3
;
Shunlin PENG
4
,
5
Author Information
1. Department of Otolaryngology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China.
2. Department of Otolaryngology, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300074, China.
3. Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China.
4. Department of Otolaryngology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China. *Corresponding author, E-mail: shunlin_peng@
5. com.
- Publication Type:Journal Article
- MeSH:
Animals;
T-Lymphocytes, Regulatory/drug effects*;
Th17 Cells/drug effects*;
Gastrointestinal Microbiome/drug effects*;
Rats, Sprague-Dawley;
Drugs, Chinese Herbal/therapeutic use*;
Female;
Rhinitis, Allergic/microbiology*;
Rats;
Cytokines
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(11):961-970
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism by which She-Ti-Zhi-Qiu decoction alleviates allergic rhinitis (AR) through gut microbiota-mediated regulation of T helper cell 17(Th17)/regulatory T cells(Treg) balance and related cytokines. Methods Twenty-eight female SD rats were randomly divided into four groups: the Control group, Model group, STZQ group, and Probiotics group. Except for the Control group, all other groups were sensitized with ovalbumin (OVA) to establish AR models. The Control and Model groups received intragastric administration of normal saline, while the STZQ group was administered She-Ti-Zhi-Qiu Decoction, and Probiotics group received probiotics. After two weeks of continuous intragastric administration, nasal mucosa, serum, peripheral blood, and colon contents were collected. The inflammation of nasal mucosal tissue was assessed via HE staining. 16S rDNA sequencing was used to detect and analyze the structure and content of bacteria in colon contents. Flow cytometry was used to detect the relative proportions of Treg and Th17 cells in peripheral blood. ELISA was used to measure the levels of Th17- and Treg-related cytokines in serum. Results Compared with the Control group, the Model group showed an inflammatory response in nasal mucosal tissue, along with increased IL-17A and IL-17E levels and decreased IL-10 levels. The percentage of Th17 cells in peripheral blood increased, while the percentage of Treg cells decreased. Beneficial bacteria in the intestine were decreased, while pathogenic bacteria were increased. Compared with the Model group, the STZQ group showed lower serum IL-17A and IL-17E levels and higher IL-10 levels. The percentage of Th17 in peripheral blood decreased, while the percentage of Treg increased. There was an increase in beneficial bacteria in the intestine and a decrease in pathogenic bacteria. The changes in the microbiota were correlated with IL-17A, IL-17E, and IL-10 levels. Conclusion She-Ti-Zhi-Qiu decoction can ameliorate the inflammation of AR by regulating gut microbiota and Th17/Treg immune balance.
