Preparation and identification of a novel microparticle-loaded DC vaccine against hepatocellular carcinoma.
- Author:
Zhao ZHAN
1
;
Xuezheng LIU
2
;
Doudou DONG
3
;
Dingyu CHEN
4
;
Yaling SUN
5
Author Information
1. Department of Clinical Laboratory, The First Affiliated Hospital of Yangtze University, Jingzhou 434000; School of Basic Medicine, Fujian Medical University, Fuzhou 350122, China.
2. Department of Clinical Laboratory, The First Affiliated Hospital of Yangtze University, Jingzhou 434000, China.
3. Experimental Surgery Department, Tangdu Hospital of Air Force Medical University, Xi'an 710038, China.
4. Department of Obstetrics and Gynecology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
5. Department of Urology, The First Affiliated Hospital of Yangtze University, Jingzhou 434000, China. *Corresponding author, E-mail: 339081132@qq.com.
- Publication Type:Journal Article
- MeSH:
Carcinoma, Hepatocellular/therapy*;
Liver Neoplasms/therapy*;
Dendritic Cells/immunology*;
Humans;
Cancer Vaccines/immunology*;
CD8-Positive T-Lymphocytes/immunology*;
Cell Line, Tumor;
Tumor Necrosis Factor-alpha/immunology*;
Interferon-gamma/immunology*;
Cell-Derived Microparticles/immunology*;
Animals
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(10):913-920
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize the properties of Hepa1-6-derived microparticles (Hepa1-6-MPs), investigate their stimulatory effects on dendritic cells (DCs) and their cellular uptake pathways, and explore the specific cytotoxic effects of CD8+ T cells induced by Hepa1-6-MP-loaded DCs on hepatoma cell lines, with the aim of developing a novel immunotherapeutic model for hepatocellular carcinoma (HCC). Methods The isolated Hepa1-6-MPs were identified using Western blotting, transmission electron microscopy (TEM) and dynamic light scattering (DLS). Flow cytometry was used to assess the uptake pathways of Hepa1-6-MPs by DCs. Subsequently, enzyme-linked immunosorbent assay (ELISA) was used to measure the effects of Hepa1-6-MP-loaded DCs on the release levels of tumor necrosis factor α(TNF-α) and interferon γ(IFN-γ) into the supernatant of CD8+ T cells. Lactate dehydrogenase (LDH) tests were conducted to evaluate the cytotoxic effects of CD8+ T cells stimulated by Hepa1-6-MP-loaded DCs on hepatoma cells. Results The morphology, size and protein markers of Hepa1-6-MPs met the established criteria. Hepa1-6-MPs enhanced the expression of DC maturation markers CD80 and CD86, and were internalized by DCs via clathrin-mediated endocytosis and phagocytosis pathways. Subsequently, Hepa1-6-MP-loaded DCs stimulated CD8+ T cells to release high levels of TNF-α and IFN-γ, which induced their specific cytotoxicity against HCC cells. Conclusion These findings suggest that Hepa1-6-MP-loaded DCs may be a promising HCC immunotherapeutic tool.
