Effects of allergens on the expression of blood basophil activation markers in patients with allergic rhinitis.
- Author:
Qiuli WANG
1
;
Weihua XU
1
;
Fangqiu GU
2
;
Siqin WANG
3
;
Junling WANG
4
,
5
Author Information
1. Department of Otolaryngology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China.
2. Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, China.
3. Department of Allergy, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou 450003, China.
4. Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001; Department of Allergy, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou 450003, China. *Corresponding author, E-mail: wangjuneling@
5. com.
- Publication Type:Journal Article
- MeSH:
Basophils/metabolism*;
Humans;
Allergens/immunology*;
Animals;
Rhinitis, Allergic/blood*;
Female;
Male;
Adult;
Mice;
Biomarkers/blood*;
Tetraspanin 30/blood*;
Interleukin-4/blood*;
Interleukin-8/blood*;
Receptors, IgE/blood*;
Phosphoric Diester Hydrolases;
Young Adult;
Pyrophosphatases;
Middle Aged;
Mice, Inbred BALB C
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(9):810-817
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of blood basophil activation markers in patients with allergic rhinitis (AR) and the effects of allergens on their expression. Methods The blood samples were collected from the following four groups: healthy control (HC), AR patients with negative skin prick test (nAR), seasonal AR patients (sAR) and perennial AR patients (pAR). Flow cytometry was employed to analyze the expression of basophil activation markers Immunoglobulin E receptor I alpha(FcepsilonRIα), CD63 and CD203c in AR patients. Plasma levels of interleukin 4 (IL-4) and IL-8 were measured by liquid-phase chip technology, and their correlations with the percentages of activated basophils were further analyzed. An ovalbumin-induced AR mouse model was established, and the expression levels of FcepsilonRIα and CD63 on blood basophils were detected. Results The expression of FcepsilonRIα, CD203c and CD63 on basophils were increased in nAR, sAR and pAR patients. Allergens enhanced the mean florescence intensity expression of CD63 and CD203c on basophils of sAR and pAR patients. The plasma levels of IL-4 and IL-8 were elevated in nAR, sAR and pAR patients, showing moderate to high correlations with the expression levels of basophil activation markers. The FcepsilonRIαand CD63 expression on basophils of AR mice were increased. Conclusion Allergens may contribute to AR pathogenesis by upregulating the expression of FcepsilonRIα, CD63 and CD203c, as well as promoting the secretion of IL-4 and IL-8.
