Effects of lncRNA DHRS4-AS1 on proliferation, invasion, migration, and apoptosis of thyroid cancer cells by regulating the miR-221-3p/SOCS3 signaling axis.
- Author:
Hui WANG
1
;
Yu GUO
2
;
Peipei ZHANG
3
;
Haoyu YANG
4
;
Chuntao TIAN
5
;
Mingming JIN
6
,
7
Author Information
1. Department of Breast Surgery, Sanmenxia Central Hospital, Sanmenxia 472000, China.
2. Pathology Department, Sanmenxia Central Hospital, Sanmenxia 472000, China.
3. Department of Radiotherapy, Sanmenxia Central Hospital, Sanmenxia 472000, China.
4. Ultrasound Department, Sanmenxia Central Hospital, Sanmenxia 472000, China.
5. Department of Oncology, Sanmenxia Central Hospital, Sanmenxia 472000, China.
6. General Surgery Ward 2, Sanmenxia Central Hospital, Sanmenxia 472000, China. *Corresponding author, E-mail: jmmsmxszxyygdwk@
7. com.
- Publication Type:Journal Article
- MeSH:
MicroRNAs/metabolism*;
Suppressor of Cytokine Signaling 3 Protein/metabolism*;
Humans;
RNA, Long Noncoding/metabolism*;
Apoptosis/genetics*;
Cell Proliferation/genetics*;
Cell Movement/genetics*;
Thyroid Neoplasms/physiopathology*;
Animals;
Signal Transduction/genetics*;
Cell Line, Tumor;
Mice, Nude;
Neoplasm Invasiveness;
Gene Expression Regulation, Neoplastic;
Mice;
Mice, Inbred BALB C
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(9):798-805
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influences of long-chain noncoding RNA DHRS4-AS1 (lncRNA DHRS4-AS1) on the proliferation, invasion, migration, and apoptosis of thyroid cancer (TC) cells by regulating the microRNA-221-3p (miR-221-3p)/suppressor of cytokine signaling 3 (SOCS3) signaling axis. Methods Quantitative real-time PCR (qRT-PCR) was applied to detect the expression of lncRNA DHRS4-AS1, miR-221-3p, and SOCS3 mRNA in TC cell lines, and the optimal cell line was selected for subsequent experiments. FTC-133 cells were divided into five groups: control group, pcDNA-NC group, DHRS4-AS1 group, DHRS4-AS1 combined with agomir NC group, and DHRS4-AS1 combined with miR-221-3p-agomir group. Transfection efficiency was assessed using qRT-PCR. Dual luciferase reporter assays were applied to verify the targeting interaction between lncRNA DHRS4-AS1, SOCS3, and miR-221-3p. Western blot analysis was used to detect the expression of SOCS3 in FTC-133 cells. EdU method was used to measure cell proliferation. Flow cytometry was applied to measure the apoptosis of FTC-133 cells. Scratch experiment was applied to measure the migration of FTC-133 cells. Transwell chamber was applied to detect the invasion of FTC-133 cells. Nude mouse transplantation tumor experiment was used to observe the effect of lncRNA DHRS4-AS1 on the growth of TC transplantation tumors. Results Dual luciferase reporter assays showed a targeting relationship between lncRNA DHRS4-AS1, miR-221-3p, and SOCS3. LncRNA DHRS4-AS1 and SOCS3 were downregulated and miR-221-3p was upregulated in FTC-133 cells. Overexpression of lncRNA DHRS4-AS1 inhibited proliferation, migration, and invasion of FTC-133 cells, while inducing apoptosis. Conversely, miR-221-3p overexpression reversed these inhibitory effects, and suppressed the apoptosis. Nude mouse transplantation experiment observed that overexpression of lncRNA DHRS4-AS1 resulted in a decrease in tumor tissue quality and volume, and a decrease in miR-221-3p expression and an increase in SOCS3 expression. Conclusion LncRNA DHRS4-AS1 is downregulated in FTC-133 cells. Overexpression of lncRNA DHRS4-AS1 can inhibit the proliferation, invasion, and migration of TC cells and induce apoptosis by regulating the miR-221-3p/SOCS3 signaling axis.
