The effect of gentiopicroside on osteogenic differentiation of human bone marrow mesenchymal stem cells by regulating the SDF-1/CXCR4 signaling pathway.
- Author:
Ruifang WANG
1
,
2
;
Yingchun YANG
3
;
Haibing QIAO
3
;
Ying YANG
3
Author Information
1. Anatomy Teaching and Research Office, Department of Basic Medicine, Fenyang College, Shanxi Medical University, Fenyang 032200, China. *Corresponding author, E-mail: fuyou56679@
2. com.
3. Anatomy Teaching and Research Office, Department of Basic Medicine, Fenyang College, Shanxi Medical University, Fenyang 032200, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Receptors, CXCR4/genetics*;
Mesenchymal Stem Cells/metabolism*;
Chemokine CXCL12/genetics*;
Iridoid Glucosides/pharmacology*;
Osteogenesis/drug effects*;
Cell Differentiation/drug effects*;
Signal Transduction/drug effects*;
Cells, Cultured;
Apoptosis/drug effects*;
Bone Marrow Cells/metabolism*
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(9):784-789
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of gentiopicroside on osteogenic differentiation of human bone marrow mesenchymal stem cells (BMSCs), and to determine whether its mechanism involves the stromal cell-derived factor 1(SDF-1)/C-X-C chemokine receptor 4 (CXCR4) pathway. Methods BMSCs were divided into six groups: normal culture control group, osteogenic induction model group, low-dose gentiopicroside (L-gentiopicroside, 10 μmol/L) group, medium-dose gentiopicroside (M-gentiopicroside, 20 μmol/L) group, high-dose gentiopicroside (H-gentiopicroside, 40 μmol/L) group, and H-gentiopicroside+SDF-1/CXCR4 pathway inhibitor (AMD3100) group (H-gentiopicroside+AMD3100, 40 μmol/L gentiopicroside+10 μg/mL AMD3100). Cell viability, apoptosis, ALP activity, mineralized nodule formation, and protein levels of the SDF-1/CXCR4 pathway were assessed using the CCK-8 assay, flow cytometry, ALP staining, Alizarin Red S staining, and Western blotting, respectively. Results No mineralized nodules were observed in either the control and model group, although the color of the model group deepened. Compared with the control group, the model group showed significantly increased A value, ALP activity, expression levels of Runt related transcription factor 2 (RUNX2), osteopontin (OPN), SDF-1, CXCR4 proteins, along with a lower apoptosis rate. Compared with the model group, the L-gentiopicroside, M-gentiopicroside and H-gentiopicroside groups showed dose-dependently (L
