Effects of ROCK-siRNA transfection on Ang II-induced endothelial cell senescence and endothelial microparticles.
- Author:
Kai WANG
1
;
Yan WANG
2
,
3
;
Tianqi CHEN
1
;
Fang PENG
1
;
Hui ZHOU
1
;
Qin SHI
1
Author Information
1. Department of Physiology, School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
2. Department of Physiology, School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China. *Corresponding author, E-mail: wangyanpiano@
3. com.
- Publication Type:Journal Article
- MeSH:
Humans;
Angiotensin II/pharmacology*;
Cellular Senescence/genetics*;
Human Umbilical Vein Endothelial Cells/cytology*;
RNA, Small Interfering/metabolism*;
Reactive Oxygen Species/metabolism*;
Sirtuin 1/genetics*;
Transfection;
Tumor Suppressor Protein p53/genetics*;
Cell-Derived Microparticles/drug effects*;
rho-Associated Kinases/metabolism*;
Endothelial Cells/metabolism*;
Cells, Cultured
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(9):778-783
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ROCK-siRNA transfection on endothelial cell senescence and endothelial microparticles (EMPs) induced by angiotensin II (Ang II). Methods Human umbilical vein endothelial cells (HUVECs) were treated with Ang II (1.0 μmo/L) to induce cellular senescence models, followed by transfection with ROCK-siRNA. The cells were divided into four groups: control group, model group, negative transfection control group (Ang II combined with NC-siRNA), and ROCK-siRNA transfection group (Ang II combined with ROCK-siRNA). Cellular senescence was assessed by SA-β-Gal staining. EMP levels in cell supernatants and intracellular reactive oxygen species (ROS) levels were assessed using flow cytometry. The expression levels of silenced information regulator 1(SIRT1) and p53 protein in each group were analyzed by Western blotting. Results Following ROCK-siRNA transfection, the number of senescent cells induced by Ang II was significantly reduced, accompanied by decreased CD31+ EMP levels and suppressed intracellular ROS levels. Meanwhile, the expression levels of SIRT1 were up-regulated, while the expression levels of p53 were down-regulated. Conclusion Silencing ROCK expression suppresses EMP release, reduces ROS generation, regulates the expression of SIRT1 and p53, and ultimately attenuates Ang II-induced endothelial cell senescence.
