mTOR promotes oxLDL-induced vascular smooth muscle cell ferroptosis by inhibiting autophagy.
- Author:
Yi LI
1
;
Lijun ZHANG
1
;
Yuke ZHANG
1
;
Qi ZHANG
1
;
Lijun ZHANG
2
Author Information
1. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
2. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China. *Corresponding author, E-mail: lijunwz@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Ferroptosis/drug effects*;
Lipoproteins, LDL/metabolism*;
TOR Serine-Threonine Kinases/physiology*;
Autophagy/drug effects*;
Muscle, Smooth, Vascular/metabolism*;
Animals;
Rats;
Myocytes, Smooth Muscle/cytology*;
Cells, Cultured;
Lipid Peroxidation/drug effects*;
Sequestosome-1 Protein/genetics*;
Phospholipid Hydroperoxide Glutathione Peroxidase/metabolism*;
Microtubule-Associated Proteins/genetics*;
Sirolimus/pharmacology*
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(8):687-694
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role and mechanism of mammalian target of rapamycin (mTOR) in oxidized low-density lipoprotein (oxLDL)-induced ferroptosis in vascular smooth muscle cells (VSMCs). Methods A model of oxLDL-induced VSMC ferroptosis was established. VSMCs were co-treated with either the mTOR inhibitor rapamycin or the autophagy inducer carbonyl cyanide m-chlorophenylhydrazone (CCCP), followed by detection of autophagy and ferroptosis-related indexes. Quantitative real-time PCR and Western blot were used respectively to analyze the expression of mTOR, glutathione peroxidase 4 (GPX4), sequestosome 1 (p62), and microtubule-associated protein 1 light chain 3 (LC3). Flow cytometry was employed to assess VSMC death. C11 BODIPY fluorescent staining was used to measure cellular lipid peroxidation levels. Colorimetric assays were performed to determine the contents of malondialdehyde (MDA), ferrous ion (Fe2+) and glutathione (GSH). Results oxLDL significantly upregulated mTOR expression in VSMCs, while increasing p62 expression and reducing LC3 expression, thereby suppressing VSMC autophagy. Compared with oxLDL treatment alone, rapamycin co-treatment reversed oxLDL-induced VSMC ferroptosis, as characterized by reduced VSMC death, increased GPX4 expression and GSH contents, along with decreased MDA content, Fe2+ content and lipid peroxidation levels. Similarly, CCCP co-treatment activated autophagy characterized by reduced p62 expression and elevated LC3 expression, which subsequently alleviated oxLDL-induced ferroptosis, showing reduced VSMC death, increased GPX4 expressions and GSH contents, and decreased MDA content, Fe2+ content and lipid peroxidation levels. Moreover, mTOR inhibition by rapamycin significantly reversed the oxLDL-induced upregulation of p62 and downregulation of LC3. Conclusion mTOR may promote oxLDL-induced VSMC ferroptosis by suppressing autophagy.
